Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	227,039	Δ1 bp	noncoding (1281/2904 nt)	rrlH →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	227,037	0	G	.	100.0%	45.6 / NA	13	noncoding (1279/2904 nt)	rrlH	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (6/7);  total (6/7)

TAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCA  >  NC_000913/226961‑227065                                                                             |                             tAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCggtga                          <  1:53815/81‑4 (MQ=255) tAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCggtga                          <  2:53816/81‑4 (MQ=255)    gcttgtgAAGGTGTACTGTGAGGTATGCTGGAGGTATTAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGaaaa                      <  2:53817/78‑1 (MQ=14)      cTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGc                    >  1:53866/1‑81 (MQ=255)       tgtgAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGcc                   >  1:53869/1‑81 (MQ=255)          gAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGc                >  2:53858/1‑81 (MQ=255)           aaGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCt               >  1:53864/1‑81 (MQ=255)           aaGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCt               >  2:53862/1‑81 (MQ=255)            aGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCTc              >  1:53868/1‑81 (MQ=255)               tgtACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCTCGcc           <  1:53818/81‑1 (MQ=255)               tgtACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCTCGcc           <  1:53819/81‑1 (MQ=255)                   cTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCTCGCCGGa        <  2:53820/80‑1 (MQ=255)                      tgAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCTCGCCGGAAGa     <  1:53821/80‑1 (MQ=255)                      tgAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCTCGCCGGAAGa     <  2:53822/80‑1 (MQ=255)                        aGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGC‑GGTGAAAAGCCCGCTCGCCGGAAGACCa  <  1:53823/81‑1 (MQ=16)                                                                             |                             TAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCA  >  NC_000913/226961‑227065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑