Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,771	Δ1 bp	intergenic (+147/‑167)	asnT → / → yeeJ	tRNA‑Asn/inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,768	0	C	.	100.0%	71.4 / NA	18	intergenic (+144/‑170)	asnT/yeeJ	tRNA‑Asn/inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/10);  total (8/10)

GGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACG  >  NC_000913/2044688‑2044838                                                                                 |                                                                       ggATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTGTc                                                                        >  2:363047/1‑81 (MQ=255)       cGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccctcga                                                                  <  2:363026/81‑5 (MQ=255)         ggTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGAtt                                                               <  1:363027/81‑1 (MQ=255)           tttttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATtat                                                             <  1:363028/81‑1 (MQ=255)                   aTCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACtt                                                      >  1:363055/1‑80 (MQ=255)                   aTCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTg                                                     <  1:363029/81‑1 (MQ=255)                       tAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGt                                                  >  1:363045/1‑80 (MQ=255)                        aaGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGa                                                    <  1:363031/77‑1 (MQ=255)                        aaGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGtt                                                 <  1:363030/80‑1 (MQ=255)                                  gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCagtagt                                       <  2:363032/80‑1 (MQ=255)                                  gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTGGtc                                      >  2:363050/1‑81 (MQ=255)                                  gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGtc                                      >  1:363052/1‑81 (MQ=255)                                     cTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTcc                                   <  2:363033/81‑1 (MQ=255)                                        tATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGA‑TATAAAACTTGAGTTATTCAGTAGTCTCCCctc                               >  2:363046/1‑81 (MQ=255)                                                aGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAAc                        >  2:363056/1‑81 (MQ=255)                                                  ggCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTc                      <  2:363034/81‑1 (MQ=255)                                                          gCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCa               >  2:363051/1‑80 (MQ=255)                                                           cTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCaa              <  2:363035/80‑1 (MQ=255)                                                                aaCGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc        >  2:363057/1‑81 (MQ=255)                                                                      aTCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACg  <  2:363036/81‑1 (MQ=255)                                                                                 |                                                                       GGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACG  >  NC_000913/2044688‑2044838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑