Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	231,190	Δ1 bp	coding (69/801 nt)	yafD →	endonuclease/exonuclease/phosphatase domain‑containing protein YafD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	231,187	0	G	.	100.0%	93.7 / NA	23	coding (66/801 nt)	yafD	endonuclease/exonuclease/phosphatase domain‑containing protein YafD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (16/7);  total (16/7)

TTTCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATT  >  NC_000913/231108‑231253                                                                                |                                                                   tttCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCgg                                                                   <  2:54736/81‑1 (MQ=255)     aaaGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCgggtct                                                               <  2:54738/81‑4 (MQ=255)     aaaGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCgggtct                                                               <  2:54737/81‑4 (MQ=255)      aaGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCgggtctt                                                              <  1:54739/81‑5 (MQ=255)      aaGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCgggtctt                                                              >  1:54761/1‑77 (MQ=255)       aGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCttt                                                            >  2:54800/1‑81 (MQ=255)           aaaaGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGc                                                         >  2:54763/1‑80 (MQ=255)              aGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAg                                                      >  1:54768/1‑80 (MQ=255)               gTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGTAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCa                                                    >  2:54760/1‑81 (MQ=255)                 gCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATc                                                  >  1:54773/1‑81 (MQ=255)                  cGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATc                                                  <  2:54740/80‑1 (MQ=255)                  cGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATc                                                  <  2:54741/80‑1 (MQ=255)                     aaaaaCACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGc                                               >  2:54783/1‑80 (MQ=255)                      aaaaCACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGc                                               >  2:54782/1‑79 (MQ=255)                         acacCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAgg                                           >  1:54776/1‑80 (MQ=255)                         acacCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAgg                                           >  2:54778/1‑80 (MQ=255)                                tGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTacca                                   <  1:54742/81‑1 (MQ=255)                                   cATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCt                                <  2:54743/81‑1 (MQ=255)                                    aTGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTg                               >  2:54775/1‑81 (MQ=255)                                     tGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTgg                              <  1:54744/81‑1 (MQ=255)                                      gcgcTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTggg                             >  1:54786/1‑81 (MQ=255)                                        gcTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTgggg                            >  1:54769/1‑80 (MQ=255)                                              ttGCCGGACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGt                      <  1:54745/80‑1 (MQ=255)                                                    gACAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCTAGGCATTACCACCTGGGGAACCGTTAAGTAc               >  2:54781/1‑81 (MQ=255)                                                     aCAACCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTAcc              >  1:54780/1‑81 (MQ=255)                                                       aaCCTGCGGAAAGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCg             >  2:54772/1‑80 (MQ=255)                                                                aaaGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTAt   >  1:159773/1‑81 (MQ=255)                                                                 aaGGATCTTACCGCC‑GGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTAtt  <  1:54746/81‑1 (MQ=255)                                                                                |                                                                   TTTCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATT  >  NC_000913/231108‑231253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑