Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,087,287	(G)7→6	intergenic (‑173/+42)	yoeI ← / ← yeeY	uncharacterized protein YoeI/putative DNA‑binding transcriptional regulator YeeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,087,281	0	G	.	100.0%	67.6 / NA	17	intergenic (‑167/+48)	yoeI/yeeY	uncharacterized protein YoeI/putative DNA‑binding transcriptional regulator YeeY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/8);  total (9/8)

AGCAAAAAATAACCGACGCTGATGAAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAATGGGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTAAATGGCTGATTTTCCGTC  >  NC_000913/2087208‑2087349                                                                          |                                                                     aGCAAAAAATAACCGACGCTGATGAAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAATgggggga                                                                >  2:369979/1‑79 (MQ=255) aGCAAAAAATAACCGACGCTGATGAAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAATgggggga                                                                >  1:369971/1‑79 (MQ=255)     aaaaaTAACCGACGCTGATGAAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGa                                                          >  1:369973/1‑81 (MQ=255)         aTAACCGACGCTGATGAAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGAc                                                         >  1:369970/1‑78 (MQ=255)             ccGACGCTGATGAAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGaa                                                  <  2:369950/81‑1 (MQ=255)                 cGCTGATGAAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATgag                                              <  2:369951/81‑1 (MQ=255)                       tgaAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCtt                                        <  1:369952/81‑1 (MQ=255)                              tCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGaa                                 <  2:369953/81‑1 (MQ=255)                                  tttttAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGaataat                             >  2:369976/1‑81 (MQ=255)                                        gTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTc                        <  1:369954/80‑1 (MQ=255)                                        gTCATTTTTTGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCt                       <  1:369955/81‑1 (MQ=255)                                             tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTaaa                  >  1:369992/1‑81 (MQ=255)                                              ttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTTAGAATAATTTTTCTTTAAAt                 >  1:369978/1‑81 (MQ=255)                                                   aCAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTAAATGGCt             >  1:369974/1‑80 (MQ=255)                                                      gcggcgCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTAAATGGCTGAtt         >  1:369980/1‑81 (MQ=255)                                                       cggcgCATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTAAATGGCTGAttt        >  1:369977/1‑81 (MQ=255)                                                          cgcATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTAAATGGCTGATTTTcc     >  2:369986/1‑81 (MQ=255)                                                           gcATTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTAAATGGCTGATTTTCCg    <  1:369956/81‑1 (MQ=255)                                                             aTTGTGCCTAAAT‑GGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTAAATGGCTGATTTTCCGtc  <  1:369957/81‑1 (MQ=255)                                                                          |                                                                     AGCAAAAAATAACCGACGCTGATGAAACGTCGGTTTTTAGTCATTTTTTGACAGCGGCGCATTGTGCCTAAATGGGGGGGAAATGACAAGAGAATGAGAGGCTTGTCAGAATAATTTTTCTTTAAATGGCTGATTTTCCGTC  >  NC_000913/2087208‑2087349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑