Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	238,652	(A)6→5	coding (85/480 nt)	ykfM ←	uncharacterized protein YkfM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	238,647	0	A	.	100.0%	27.9 / NA	8	coding (90/480 nt)	ykfM	uncharacterized protein YkfM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/3);  total (5/3)

GAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATT  >  NC_000913/238574‑238720                                                                          |                                                                          gAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaca                                                                     >  1:55966/1‑78 (MQ=255)   aTCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAACAaag                                                                 <  2:55959/81‑1 (MQ=255)         aTTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAACAAAGAAGCAt                                                           >  2:55975/1‑81 (MQ=255)               cAATCATAAAATATATCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAACAAAGAAGCATTGAAt                                                      <  1:55960/80‑1 (MQ=255)                              tCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAACAAAGAAGCATTGAATGCAGGGAAAAataata                                      >  1:55976/1‑81 (MQ=255)                               ccGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAACAAAGAAGCATTGAATGCAGGGAAAAATAatat                                     >  2:55969/1‑81 (MQ=255)                                                attGAATATAGAGGTTTAACTCCGGT‑AAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATc                     <  1:55961/80‑1 (MQ=255)                                                                tAACTCCGGT‑AAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTtttaa    >  1:55983/1‑81 (MQ=255)                                                                   cTCCGGT‑AAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTtttaatt  >  2:55980/1‑80 (MQ=255)                                                                          |                                                                          GAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATT  >  NC_000913/238574‑238720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑