Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,183,325	Δ1 bp	coding (327/819 nt)	yegX ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,183,322	0	G	.	100.0%	89.9 / NA	22	coding (330/819 nt)	yegX	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/9);  total (13/9)

CCGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTTTTG  >  NC_000913/2183246‑2183398                                                                             |                                                                             ccGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAgggtc                                                                          >  2:386492/1‑79 (MQ=255)   gACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAgggtcca                                                                        >  1:386509/1‑77 (MQ=255)          agaAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCtt                                                                <  1:386465/81‑1 (MQ=255)             aataatGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTc                                                             <  2:386466/81‑1 (MQ=255)                  tGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCtt                                                        >  1:386502/1‑81 (MQ=255)                    aTACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTcc                                                      <  2:386467/81‑1 (MQ=255)                     tACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCg                                                     >  1:386497/1‑81 (MQ=255)                                cagcagGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTtaata                                          <  2:386468/81‑1 (MQ=255)                                  gcatGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTtaataa                                         >  1:386552/1‑80 (MQ=255)                                    agGCCATTTTCGCGGCTTAGTTTCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAg                                      >  2:386511/1‑81 (MQ=255)                                    agGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAg                                      >  1:386505/1‑81 (MQ=255)                                               gcgGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAg                           >  1:386482/1‑81 (MQ=255)                                                cgGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGc                          <  2:386469/81‑1 (MQ=255)                                                       gTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATAc                    >  1:386501/1‑80 (MQ=255)                                                       gTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTCAATAAAAGCAAACTGTAAGCGGATAc                    <  2:386470/80‑1 (MQ=255)                                                          gCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCAtt                >  1:386513/1‑81 (MQ=255)                                                             aGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTc             >  1:386514/1‑81 (MQ=255)                                                             aGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTc             <  2:386471/81‑1 (MQ=255)                                                             aGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTc             >  2:386507/1‑81 (MQ=255)                                                               ttACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTcgc           <  2:386472/81‑1 (MQ=255)                                                                 aCGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTcgcg          >  2:386504/1‑80 (MQ=255)                                                                  cgcgAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCgcgc         <  2:386473/80‑1 (MQ=255)                                                                   gcgAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCAt       >  1:386521/1‑81 (MQ=255)                                                                        aaaTA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTTTTg  >  1:386500/1‑81 (MQ=255)                                                                             |                                                                             CCGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTTTTG  >  NC_000913/2183246‑2183398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑