Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,225,698	(T)6→5	intergenic (‑67/‑103)	yohC ← / → yohD	putative inner membrane protein/DedA family protein YohD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,225,693	0	T	.	100.0%	80.5 / NA	20	intergenic (‑62/‑108)	yohC/yohD	putative inner membrane protein/DedA family protein YohD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/8);  total (12/8)

TCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAGGGTGTAT  >  NC_000913/2225614‑2225766                                                                                |                                                                          tCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattatt                                                                          <  2:393783/81‑1 (MQ=255)      aGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAtttttgt                                                                     >  1:393816/1‑79 (MQ=255)        aCATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAtttttgttc                                                                   <  2:393784/81‑5 (MQ=255)                 aTACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGt                                                          <  1:393785/80‑1 (MQ=255)                 aTACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTa                                                         <  1:393786/81‑1 (MQ=255)                   aCCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAAt                                                       >  2:393812/1‑81 (MQ=255)                         cccTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCAc                                                 >  2:393815/1‑81 (MQ=255)                           cTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTg                                               <  1:393787/81‑1 (MQ=255)                                    aGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCtgatga                                       <  1:393788/80‑1 (MQ=255)                                    aGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGaa                                      >  1:393817/1‑81 (MQ=255)                                          tGAACCTGACAGTTAAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCt                                <  1:393789/81‑1 (MQ=255)                                                 gACAGTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAg                         >  1:393820/1‑81 (MQ=255)                                                     gTTCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGt                      >  2:393818/1‑80 (MQ=255)                                                      ttCAAGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTg                     >  1:393819/1‑80 (MQ=255)                                                         aaGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAAt                  >  1:393829/1‑80 (MQ=255)                                                         aaGTATAAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAAt                  <  2:393790/80‑1 (MQ=255)                                                            tataAGTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGAtt              >  1:393824/1‑81 (MQ=255)                                                                 gTCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAg         >  1:393823/1‑81 (MQ=255)                                                                  tCAGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAgg        <  1:393791/81‑1 (MQ=255)                                                                    aGCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAGGgt      >  1:393835/1‑81 (MQ=255)                                                                     gCTTGTGATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAGGgtg     >  1:393833/1‑81 (MQ=255)                                                                        tgtgATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAGGGTGTAt  >  2:393842/1‑81 (MQ=255)                                                                         gtgATTA‑TTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAGGGTGTa   <  2:393792/79‑1 (MQ=255)                                                                                |                                                                          TCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAGGGTGTAT  >  NC_000913/2225614‑2225766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑