Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,285,152	Δ1 bp	coding (777/1761 nt)	yejM →	putative cardiolipin transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,285,150	0	G	.	100.0%	103.0 / NA	25	coding (775/1761 nt)	yejM	putative cardiolipin transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/16);  total (9/16)

GTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTG  >  NC_000913/2285070‑2285221                                                                                 |                                                                        gTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCg                                                                         <  1:404569/81‑1 (MQ=255)  tCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCgg                                                                        <  2:404570/81‑1 (MQ=255)    ttATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCgg                                                                        >  2:404615/1‑79 (MQ=255)     tATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCgg                                                                        >  2:404614/1‑78 (MQ=255)               ggTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGt                                                          >  1:404621/1‑81 (MQ=255)                  aaTCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGa                                                       >  2:404620/1‑81 (MQ=255)                    tCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGAtt                                                     <  2:404571/81‑1 (MQ=255)                       aGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTaat                                                  <  1:404572/81‑3 (MQ=255)                             cGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGAt                                            <  2:404573/81‑1 (MQ=255)                                   cGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCt                                       <  2:404574/80‑1 (MQ=255)                                   cGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTg                                      <  1:404576/81‑1 (MQ=255)                                   cGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTg                                      <  2:404575/81‑1 (MQ=255)                                       cAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAac                                   <  1:404577/80‑1 (MQ=255)                                       cAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAac                                   >  2:404612/1‑80 (MQ=255)                                        aGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAact                                  <  1:404578/80‑1 (MQ=255)                                         gTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAactac                                <  2:404579/81‑1 (MQ=255)                                           aTCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAactact                               >  1:404627/1‑80 (MQ=255)                                            tCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTc                              <  1:404580/80‑1 (MQ=255)                                                      gAACTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCgag                    >  1:404634/1‑80 (MQ=255)                                                       aaCTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCgag                    >  1:404625/1‑79 (MQ=255)                                                       aaCTGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCgag                    >  2:404617/1‑79 (MQ=255)                                                          tGCGCTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAg                 >  2:404624/1‑79 (MQ=255)                                                            cgcTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAg              <  2:404581/80‑1 (MQ=255)                                                             gcTATCGCGATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGAt            <  1:404582/81‑1 (MQ=255)                                                                   gcgATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTgc      <  2:404583/81‑1 (MQ=255)                                                                    cgATATGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTgcg     <  1:404584/81‑1 (MQ=255)                                                                      atatGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCt   >  1:404616/1‑81 (MQ=255)                                                                       tatGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTg  <  2:404586/81‑1 (MQ=255)                                                                       tatGGGCACC‑GGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTg  <  2:404585/81‑1 (MQ=255)                                                                                 |                                                                        GTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTG  >  NC_000913/2285070‑2285221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑