Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,310,170	Δ1 bp	coding (236/1065 nt)	ada ←	DNA‑binding transcriptional dual regulator/DNA repair protein Ada

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,310,168	0	G	.	100.0%	102.2 / NA	25	coding (238/1065 nt)	ada	DNA‑binding transcriptional dual regulator/DNA repair protein Ada
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/7);  total (18/7)

GGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTA  >  NC_000913/2310088‑2310243                                                                                 |                                                                            ggCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTg                                                                             >  2:409453/1‑81 (MQ=255)           tCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCtt                                                                  >  2:409445/1‑81 (MQ=255)             cAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCttt                                                                 <  2:409420/80‑1 (MQ=255)              aGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCttttt                                                               >  2:409452/1‑81 (MQ=255)                 gTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTg                                                            <  1:409421/81‑1 (MQ=255)                 gTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTg                                                            >  2:409447/1‑81 (MQ=255)                    aCAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGc                                                          <  2:409422/80‑1 (MQ=255)                     cAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCt                                                         >  1:409474/1‑80 (MQ=255)                     cAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCt                                                         <  2:409423/80‑1 (MQ=255)                           tttCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACg                                                  <  2:409424/81‑1 (MQ=255)                            ttCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGt                                                 >  2:409461/1‑81 (MQ=255)                              ccTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGtt                                                <  1:409425/80‑1 (MQ=255)                                  ttCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAg                                           >  1:409467/1‑81 (MQ=255)                                             gACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGc                                 >  1:409462/1‑80 (MQ=255)                                             gACACGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGc                                 >  2:409456/1‑80 (MQ=255)                                               cacGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAg                              >  2:409470/1‑81 (MQ=255)                                                acGCGTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgc                             >  2:409478/1‑81 (MQ=255)                                                  gcgTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcgg                           >  2:409469/1‑81 (MQ=255)                                                    gTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcggc                          >  1:409472/1‑80 (MQ=255)                                                    gTGGGTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcggc                          >  2:409479/1‑80 (MQ=255)                                                    gTGGGTGATCTCATCCAACCGATGTTGCT‑GGCATTGGCTTTTTTTGGCTGACAACGTTCGCAGGGGCGAAAGCCAgcggc                          >  2:409466/1‑80 (MQ=255)                                                      gggTGATTTTATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAg                       >  2:409465/1‑81 (MQ=255)                                                             tttATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCg                >  1:409471/1‑81 (MQ=255)                                                               tATCCAACCGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCt              >  1:409463/1‑81 (MQ=255)                                                                       cGATGTTGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTg      >  2:409460/1‑81 (MQ=255)                                                                            ttGCT‑GGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTa  <  1:409426/80‑1 (MQ=255)                                                                                 |                                                                            GGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTA  >  NC_000913/2310088‑2310243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑