| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,360,846 | (C)5→4 | coding (569/1206 nt) | rhmD ← | L‑rhamnonate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,360,842 | 0 | C | . | 94.1% | 60.0 / ‑2.9 | 17 | coding (573/1206 nt) | rhmD | L‑rhamnonate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (9/7); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGTGGCGTAG > NC_000913/2360763‑2360917 | aGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGcc < 1:418954/81‑1 (MQ=255)aGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGcc < 2:418953/81‑1 (MQ=255) gAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGccc < 2:418955/81‑1 (MQ=255) aaTCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGcccca < 1:418956/81‑2 (MQ=255) aTCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGccccag < 2:418957/81‑3 (MQ=255) ccTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGccccagtg > 2:419004/1‑77 (MQ=255) cTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGccccagtg < 2:418959/80‑5 (MQ=255) cTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGc < 1:418958/81‑1 (MQ=255) cATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCAcc > 1:419001/1‑80 (MQ=255) atatCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGa > 2:419013/1‑81 (MQ=255) cgcgACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAg > 1:419003/1‑81 (MQ=255) gACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGccc < 2:418960/81‑1 (MQ=255) gACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGcc > 1:418998/1‑80 (MQ=255) aTAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCAtct > 2:419000/1‑81 (MQ=255) gCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATctct < 2:418961/80‑1 (MQ=255) gCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCtt > 2:419009/1‑81 (MQ=255) cATCTTTGCGGATCACCGCATCGCCATCATGTGGCCCCCAGTGAGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTg < 1:418962/81‑1 (MQ=255) cTTTGCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGc > 2:418994/1‑78 (MQ=255) gCGGATCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATc < 1:418963/80‑1 (MQ=255) aTCCCCGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGAc < 2:418964/81‑1 (MQ=255) ccccGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGAcgc > 2:419011/1‑81 (MQ=255) cccGCATCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGAcgc < 1:418965/80‑1 (MQ=255) tCGCCATCATGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCtg > 1:419007/1‑81 (MQ=255) atGTGG‑CCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGTGGCGTAg < 1:418966/81‑1 (MQ=255) | AGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCCATCATGTGGCCCCCAGTGCGTCGGCATTTTGCCACCGATAAAGCCCATCTCTTTTGCCAGATCCGGACGCGCACCTGTGGCGTAG > NC_000913/2360763‑2360917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |