BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,443,666	Δ1 bp	coding (1903/2007 nt)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,443,666	0	A	.	100.0%	93.4 / NA	23	coding (1903/2007 nt)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (10/13); total (10/13)

TTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATT  >  NC_000913/2443592‑2443739

ttATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑Gcgacg                                                                     >  1:434681/1‑80 (MQ=255)

ttATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑Gcgacg                                                                     >  2:434695/1‑80 (MQ=255)

 tATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑Gcgacga                                                                    <  2:434646/80‑1 (MQ=255)

 tATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑Gcgacg                                                                     <  1:434647/79‑1 (MQ=255)

   tGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAAcc                                                                 <  1:434648/81‑1 (MQ=255)

        gCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTc                                                            >  1:434701/1‑81 (MQ=255)

         cGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCTACGAACCGATTcc                                                           <  1:434651/81‑1 (MQ=255)

         cGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTcc                                                           <  1:434649/81‑1 (MQ=255)

         cGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTc                                                            <  1:434650/80‑1 (MQ=255)

                   ttCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGcc                                                 <  2:434652/81‑1 (MQ=255)

                         cgGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACg                                           <  2:434653/81‑1 (MQ=255)

                          ggTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGc                                          >  2:434699/1‑81 (MQ=255)

                               gtgtTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTggcg                                     >  2:434696/1‑81 (MQ=255)

                               gtgtTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTggcg                                     >  2:434687/1‑81 (MQ=255)

                                    cTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGt                                 >  2:434697/1‑80 (MQ=255)

                                       ccccGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTaa                              <  2:434654/80‑1 (MQ=255)

                                        cccGCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAAc                            <  2:434655/81‑1 (MQ=255)

                                           gCTGTGTGCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAAccc                          >  2:434706/1‑80 (MQ=255)

                                               tgtgCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAAt                      <  1:434656/80‑1 (MQ=255)

                                                 tgCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCgg                   <  1:434658/81‑1 (MQ=255)

                                                 tgCCGAGATTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCg                    <  2:434657/80‑1 (MQ=255)

                                                       gaTTCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATgg             >  1:434689/1‑81 (MQ=255)

                                                          tCTGGCGGCGCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGTTg          >  2:434704/1‑81 (MQ=255)

                                                              gcggcgCAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGc         <  2:434659/78‑1 (MQ=255)

                                                                 gcgcAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAAt   >  2:434710/1‑81 (MQ=255)

                                                                  cgcAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAAtt  <  2:434660/81‑1 (MQ=255)

                                                                  cgcAGATG‑GCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAAtt  >  2:434698/1‑81 (MQ=255)

TTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATT  >  NC_000913/2443592‑2443739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`