Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,479,522	(A)6→5	coding (321/1329 nt)	dsdA →	D‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,479,517	0	A	.	100.0%	80.4 / NA	20	coding (316/1329 nt)	dsdA	D‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/9);  total (11/9)

AGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGCACACGC  >  NC_000913/2479438‑2479590                                                                                |                                                                          aGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGa                                                                           <  2:440600/80‑1 (MQ=255)   aaCTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGaaaa                                                                        <  1:440601/81‑1 (MQ=255)       ggTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGaaaaaga                                                                     >  1:440645/1‑78 (MQ=255)             cATTCCAGCTATGCAAAATCGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCAt                                                             <  1:440602/81‑1 (MQ=255)               ttCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCAttt                                                           <  2:440603/81‑1 (MQ=255)                 ccAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGc                                                         >  1:440625/1‑81 (MQ=255)                  cAGCTATGCAAAAACGGCTGGAAAAATAATATCAGCAACCGATCAGCGGTCAACTGTTACTG‑AAAAAGATAGCCATTTGcc                                                        >  1:440632/1‑81 (MQ=255)                   aGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGccc                                                       <  2:440604/81‑1 (MQ=255)                     cTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCAt                                                     <  2:440605/81‑1 (MQ=255)                              aaCGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTc                                            >  1:440623/1‑81 (MQ=255)                                       aaaaaGAATATCAGCAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAg                                    >  1:440638/1‑80 (MQ=255)                                                  cAGCAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGa                        >  2:440630/1‑81 (MQ=255)                                                     cAACCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGAtt                      >  2:440637/1‑80 (MQ=255)                                                       aCCGATCAGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTAt                   <  1:440606/81‑1 (MQ=255)                                                              aGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTcc            <  2:440607/81‑1 (MQ=255)                                                              aGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTcc            >  2:440636/1‑81 (MQ=255)                                                              aGCGGGCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTc             <  1:440608/80‑1 (MQ=255)                                                                  ggCAACTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGc        <  2:440609/81‑1 (MQ=255)                                                                     aaCTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGcaca     >  1:440653/1‑81 (MQ=255)                                                                     aaCTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGcaca     >  1:440634/1‑81 (MQ=255)                                                                      aCTGTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGCacac    >  1:440642/1‑81 (MQ=255)                                                                         gTTACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGCACACg   >  2:440640/1‑79 (MQ=255)                                                                          ttACTG‑AAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGCACACGc  <  1:440610/79‑1 (MQ=255)                                                                                |                                                                          AGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGCACACGC  >  NC_000913/2479438‑2479590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑