Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,483,477	(A)8→7	intergenic (‑138/‑278)	emrK ← / → evgA	tripartite efflux pump membrane fusion protein EmrK/DNA‑binding transcriptional activator EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,470	0	A	.	100.0%	32.4 / NA	9	intergenic (‑131/‑285)	emrK/evgA	tripartite efflux pump membrane fusion protein EmrK/DNA‑binding transcriptional activator EvgA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/5);  total (4/5)

ATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTGGCTTTTGCAATACAATTCTTACGCCTGTAGGATTAGTAAGAAGACTTA  >  NC_000913/2483395‑2483545                                                                            |                                                                            attcTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCaaaaaa                                                                        <  1:441206/81‑1 (MQ=255)        atatCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGC‑AAAAAAacaaca                                                                 <  1:441207/80‑1 (MQ=255)                cacaGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGC‑AAAAAAACAACAGAGGACTa                                                         >  1:441229/1‑80 (MQ=255)                   aGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGC‑AAAAAAACAACAGAGGACTAAAcc                                                     <  1:441208/81‑1 (MQ=255)                             ttGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGC‑AAAAAAACAACAGAGGACTAAACCGTGGCTTTTg                                           <  2:441209/81‑1 (MQ=255)                             ttGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGC‑AAAAAAACAACAGAGGACTAAACCGTGGCTTTTg                                           >  2:441224/1‑81 (MQ=255)                               gCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGC‑AAAAAAACAACAGAGGACTAAACCGTGGCTTTTGc                                          <  2:441210/80‑1 (MQ=255)                                               aCGTTGGAGGCCTAATTTAAATGAGGTGC‑AAAAAAACAACAGAGGACTAAACCGTGGCTTTTGCAATACAATTCTTACGcc                         >  1:441225/1‑81 (MQ=255)                                                             aTTTAAATGAGGTGC‑AAAAAAACAACAGAGGACTAAACCGTGGCTTTTGCAATACAATTCTTACGCCTGTAGGATTAGTa            <  1:441211/80‑1 (MQ=255)                                                                      aGGTGC‑AAAAAAACAACAGAGGACTAAACCGTGGCTTTTGCAATACAATTCTTACGCCTGTAGGATTAGTAAGAAGACTta  >  2:441235/1‑81 (MQ=255)                                                                            |                                                                            ATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTGGCTTTTGCAATACAATTCTTACGCCTGTAGGATTAGTAAGAAGACTTA  >  NC_000913/2483395‑2483545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑