Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,517,367	(A)5→4	coding (466/2190 nt)	pdeA ←	putative c‑di‑GMP phosphodiesterase PdeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,517,363	0	A	.	100.0%	141.7 / NA	34	coding (470/2190 nt)	pdeA	putative c‑di‑GMP phosphodiesterase PdeA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/17);  total (17/17)

GAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCGGCG  >  NC_000913/2517284‑2517438                                                                                |                                                                            gAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaa                                                                            <  2:447139/81‑1 (MQ=255)       cATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaaggtg                                                                      >  2:447171/1‑77 (MQ=255)       cATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaaggtg                                                                      >  1:447188/1‑77 (MQ=255)        aTGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTgg                                                                    <  2:447140/81‑1 (MQ=255)             gtAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGatat                                                                <  1:447142/80‑1 (MQ=255)             gtAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGatat                                                                <  2:447141/80‑1 (MQ=255)              tAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATc                                                               <  2:447143/80‑1 (MQ=255)               aaaTCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCtt                                                             >  2:447196/1‑81 (MQ=255)               aaaTCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCtt                                                             >  2:447179/1‑81 (MQ=255)                    aGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCg                                                        <  2:447144/81‑1 (MQ=255)                    aGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCg                                                        >  1:447200/1‑81 (MQ=255)                      cacaGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCggg                                                      <  2:447145/81‑1 (MQ=255)                       acaGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGa                                                     <  1:447146/81‑1 (MQ=255)                           cGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATc                                                 <  2:447147/81‑1 (MQ=255)                               gtaaaGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCa                                                >  2:447194/3‑78 (MQ=255)                               gAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCa                                                >  2:447187/1‑78 (MQ=255)                                aaaaGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAaaga                                            <  2:447148/81‑1 (MQ=255)                                 aaaGGCTTAGTAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAaagaa                                           <  2:447149/81‑1 (MQ=255)                                  aaGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAaagaag                                          >  2:447185/1‑81 (MQ=255)                                    ggCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCt                                        <  2:447150/81‑1 (MQ=255)                                         aGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCca                                   >  2:447192/1‑81 (MQ=255)                                          gCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCcac                                  <  2:447151/81‑1 (MQ=255)                                          gCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCca                                   >  1:447178/1‑80 (MQ=255)                                            aaaTCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCcac                                  >  2:447195/1‑79 (MQ=255)                                               tCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGa                              >  1:447184/1‑80 (MQ=255)                                               tCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGa                              >  2:447189/1‑80 (MQ=255)                                               tCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGAt                             <  1:447152/81‑1 (MQ=255)                                                 aaCGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATa                            >  2:447198/1‑80 (MQ=255)                                                 aaCGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATa                            >  2:447193/1‑80 (MQ=255)                                                 aaCGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATa                            >  1:447182/1‑80 (MQ=255)                                                  aCGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACa                          <  2:447153/81‑1 (MQ=255)                                                           aaaaTGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCAtt                 >  2:447172/1‑81 (MQ=255)                                                             aaTGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCAttt                >  1:447205/1‑80 (MQ=255)                                                                   aTCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGcc          >  2:447191/1‑80 (MQ=255)                                                                      cGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGAt       <  1:447154/80‑1 (MQ=255)                                                                         aTCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCGGc   <  2:447155/81‑1 (MQ=255)                                                                          tCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCGGCg  <  2:447156/81‑1 (MQ=255)                                                                                |                                                                            GAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATCGGCG  >  NC_000913/2517284‑2517438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑