Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,524,504	(C)5→4	coding (375/834 nt)	xapA ←	xanthosine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,524,500	0	C	.	100.0%	138.0 / NA	33	coding (379/834 nt)	xapA	xanthosine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (16/17);  total (16/17)

AAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTA  >  NC_000913/2524421‑2524573                                                                                |                                                                          aaaCGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCtgcctgcc                                                                          >  1:448477/1‑81 (MQ=255)       tcatcGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGccccactt                                                                    >  2:448484/1‑77 (MQ=255)        catcGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTc                                                                  >  2:448470/1‑81 (MQ=255)          tcGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCg                                                                >  2:448467/1‑81 (MQ=255)            gTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCgg                                                               <  1:448426/80‑1 (MQ=255)            gTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGa                                                              >  1:448474/1‑81 (MQ=255)             ttAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGAc                                                             <  1:448427/81‑1 (MQ=255)               aaGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACg                                                            >  1:448459/1‑80 (MQ=255)                   cccACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCg                                                       <  2:448428/81‑1 (MQ=255)                    ccaccaTCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGc                                                        >  2:448479/1‑79 (MQ=255)                         aTCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTg                                                 <  1:448429/81‑1 (MQ=255)                         aTCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTg                                                 >  2:448475/1‑81 (MQ=255)                            ggCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCg                                              >  2:448488/1‑81 (MQ=255)                                     ggCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGc                                     <  2:448430/81‑1 (MQ=255)                                          ggTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGaa                                 >  1:448482/1‑80 (MQ=255)                                           gtgtTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGaa                                 >  1:448490/1‑79 (MQ=255)                                           gtgtTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAAc                                <  2:448431/80‑1 (MQ=255)                                            tgtTGATATGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAg                              >  2:448476/1‑81 (MQ=255)                                                  tatGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTc                        <  1:448432/81‑1 (MQ=255)                                                    tGATCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCg                       <  1:448433/80‑1 (MQ=255)                                                      aTCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCa                     <  2:448434/80‑1 (MQ=255)                                                      aTCTTTCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCa                     <  1:448435/80‑1 (MQ=255)                                                          ttCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGcc                  >  1:448492/1‑79 (MQ=255)                                                           tCAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGcc                  <  1:448436/78‑1 (MQ=255)                                                            cAATGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCcag               <  2:448437/80‑1 (MQ=255)                                                             aaTGCGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCcagc              <  2:448438/80‑1 (MQ=255)                                                                 cGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCtt         <  2:448439/81‑1 (MQ=255)                                                                 cGACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCt          <  2:448440/80‑1 (MQ=255)                                                                  gACCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTa        <  1:448441/81‑1 (MQ=255)                                                                   aCCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTa        >  2:448480/1‑80 (MQ=255)                                                                   aCCAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTa        >  2:448485/1‑80 (MQ=255)                                                                    ccAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTaaa      <  1:448442/81‑1 (MQ=255)                                                                     cAGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAg     >  1:448494/1‑81 (MQ=255)                                                                      aGGCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAgg    >  1:448487/1‑81 (MQ=255)                                                                        gCTGCCTG‑CCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTa  >  2:448481/1‑81 (MQ=255)                                                                                |                                                                          AAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTA  >  NC_000913/2524421‑2524573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑