Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	278,518	Δ1 bp	coding (285/1047 nt)	afuC ←	CP4‑6 prophage; ABC transporter ATP‑binding protein AfuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	278,516	0	C	.	100.0%	98.1 / NA	24	coding (287/1047 nt)	afuC	CP4‑6 prophage; ABC transporter ATP‑binding protein AfuC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/12);  total (12/12)

GCCAACGCCTCTTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCTCCCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGAATAGAGCGAT  >  NC_000913/278438‑278588                                                                               |                                                                         gCCAACGCCTCTTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATtctctc                                                                          >  1:64631/1‑79 (MQ=255) gCCAACGCCTCTTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCTcc                                                                         <  1:64582/80‑1 (MQ=255) gCCAACGCCTCTTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCTcc                                                                         <  2:64581/80‑1 (MQ=255) gCCAACGCCTCTTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCTcc                                                                         <  2:64580/80‑1 (MQ=255)  ccAACGCCTCTTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCTccag                                                                       >  1:64632/1‑79 (MQ=255)   cAACGCCTCTTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCTccagc                                                                      <  2:64583/81‑4 (MQ=255)         ctctTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACata                                                               <  1:64584/81‑1 (MQ=255)               gACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCgg                                                          >  1:64641/1‑80 (MQ=255)                      gCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAggg                                                  <  2:64585/81‑1 (MQ=255)                           cAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAg                                             >  2:64626/1‑81 (MQ=255)                            aGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAg                                             >  1:64644/1‑80 (MQ=255)                            aGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAg                                             <  2:64586/80‑1 (MQ=255)                                 tGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTg                                        >  2:64629/1‑80 (MQ=255)                                  gcgcgcGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGa                                       >  2:64625/1‑80 (MQ=255)                                   cgcgcgGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTg                                        <  1:64587/78‑1 (MQ=255)                                   cgcgcgGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGaaa                                     >  2:64635/1‑81 (MQ=255)                                      gcgGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAacac                                  <  1:64589/81‑1 (MQ=255)                                      gcgGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAacac                                  <  1:64588/81‑1 (MQ=255)                                           aCGCCGAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATa                              >  2:64630/1‑80 (MQ=255)                                                gAGCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGata                        >  2:64636/1‑81 (MQ=255)                                                  gCATTTTCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATc                      <  2:64590/81‑1 (MQ=255)                                                        tCAGGCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCgct                 <  1:64591/80‑1 (MQ=255)                                                           ggCCATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCgctgct              >  2:64633/1‑80 (MQ=255)                                                             ccATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGaa           <  1:64593/81‑1 (MQ=255)                                                             ccATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGaa           <  2:64592/81‑1 (MQ=255)                                                              cATAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGaa           >  2:64638/1‑80 (MQ=255)                                                                tAACCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGaa           >  2:64637/1‑78 (MQ=255)                                                                 aaCCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGAATa         >  2:64649/1‑79 (MQ=255)                                                                  aCCGACATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGAATagag      <  1:64594/81‑1 (MQ=255)                                                                      aCATTCTCT‑CCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGAATAGAGCGAt  <  2:64595/81‑1 (MQ=255)                                                                               |                                                                         GCCAACGCCTCTTTGACGCGGGCTTTCAGCTCTGCGCGCGGTACGCCGAGCATTTTCAGGCCATAACCGACATTCTCTCCCAGCGACATATGCGGGAACAGGGCATAGGACTGAAACACCATACAGATATCGCGCTGCTGAATAGAGCGAT  >  NC_000913/278438‑278588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑