Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,044	Δ1 bp	coding (609/1179 nt)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,041	0	A	.	100.0%	141.5 / NA	34	coding (612/1179 nt)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/21);  total (13/21)

GCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGC  >  NC_000913/2637965‑2638117                                                                             |                                                                             gctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCaaagc                                                                          >  1:471537/1‑79 (MQ=255)  ctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCaaagc                                                                          <  1:471492/80‑3 (MQ=255)  ctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCaaagc                                                                          <  2:471493/80‑3 (MQ=255)   tgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCaaagccg                                                                        >  1:471545/1‑77 (MQ=255)    gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGt                                                                      >  2:471554/1‑81 (MQ=255)      cAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTg                                                                    >  1:471540/1‑81 (MQ=255)        aaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTc                                                                  <  1:471494/81‑1 (MQ=255)         atcatcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCTCCCCACGACGGCCGCACC‑AAAGCCGTTGTCg                                                                 >  2:471559/1‑81 (MQ=255)               tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGgcg                                                              >  1:471555/1‑78 (MQ=255)                 gCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGgcgc                                                             <  1:471495/77‑1 (MQ=255)                 gCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGa                                                          <  2:471496/80‑1 (MQ=255)                 gCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGAc                                                         <  1:471497/81‑1 (MQ=255)                       ttCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGcc                                                    <  1:471498/80‑1 (MQ=255)                          cATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGc                                                <  2:471499/81‑1 (MQ=255)                            tCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCaa                                              <  1:471500/81‑1 (MQ=255)                             cAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCa                                               >  2:471565/1‑79 (MQ=255)                              aGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCa                                               >  2:471548/1‑78 (MQ=255)                                cACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCaaa                                             >  2:471556/1‑78 (MQ=255)                                 aCTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAaga                                           >  2:471558/1‑79 (MQ=255)                                  cTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagagag                                        <  2:471501/81‑1 (MQ=255)                                    gcgcTGACGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGc                                       >  1:471561/1‑80 (MQ=255)                                          aCGCGACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGc                                 <  2:471502/80‑1 (MQ=255)                                           cgcgACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCat                               <  2:471503/81‑1 (MQ=255)                                              gACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATc                            <  2:471505/81‑1 (MQ=255)                                              gACCATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATc                            <  1:471504/81‑1 (MQ=255)                                                ccATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATc                            >  2:471536/1‑79 (MQ=255)                                                 cATTATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAg                         <  2:471506/81‑1 (MQ=255)                                                   ttATCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGt                       <  1:471507/81‑1 (MQ=255)                                                     aTCGCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGtt                      <  1:471508/80‑1 (MQ=255)                                                        gCCCCCCACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACt                  <  1:471509/81‑1 (MQ=255)                                                            cccACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTcc              <  2:471510/81‑1 (MQ=255)                                                             ccACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCa             <  1:471511/81‑1 (MQ=255)                                                              cACGACGGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCAt            >  1:471553/1‑81 (MQ=255)                                                                 gacgGCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg         <  2:471512/81‑1 (MQ=255)                                                                    ggCCGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGa        >  2:471560/1‑79 (MQ=255)                                                                       cGCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcg   <  1:471513/81‑1 (MQ=255)                                                                        gCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcgc  <  2:471514/81‑1 (MQ=255)                                                                        gCACC‑AAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcgc  >  1:471557/1‑81 (MQ=255)                                                                             |                                                                             GCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGC  >  NC_000913/2637965‑2638117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑