Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,771,880	(T)5→4	coding (275/315 nt)	yfjU ←	CP4‑57 prophage; ArsC family protein YfjU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,771,876	0	T	.	100.0%	85.0 / NA	21	coding (279/315 nt)	yfjU	CP4‑57 prophage; ArsC family protein YfjU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/5);  total (16/5)

ATCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAGGACAAGGC  >  NC_000913/2771806‑2771950                                                                       |                                                                           aTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCattaaa                                                                 <  1:499242/81‑1 (MQ=255)              cTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGatat                                                    >  1:499250/1‑81 (MQ=255)                aaGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATatg                                                   >  1:499271/1‑80 (MQ=255)                aaGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATatg                                                   >  2:499254/1‑80 (MQ=255)                 aGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATatgaa                                                 >  1:499274/1‑81 (MQ=255)                                 gATCAACCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGatat                                 >  1:499267/1‑81 (MQ=255)                                  aTCAACCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGatat                                 <  1:499244/80‑1 (MQ=255)                                  aTCAACCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGatat                                 <  2:499243/80‑1 (MQ=255)                                     aaCCATTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAAc                             <  2:499245/81‑1 (MQ=255)                                         aTTCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATgg                         >  2:499273/1‑81 (MQ=255)                                          ttCTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGc                        >  1:499255/1‑81 (MQ=255)                                            cTTGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGcc                       >  2:499281/1‑80 (MQ=255)                                             ttGATAAACCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCaa                     >  2:499272/1‑81 (MQ=255)                                                  aaaCCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATcaca                >  1:499260/1‑81 (MQ=255)                                                   aaCCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACAt               >  2:499282/1‑81 (MQ=255)                                                   aaCCCATGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACAt               >  2:499270/1‑81 (MQ=255)                                                         tGAGACGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAg          >  1:499277/1‑80 (MQ=255)                                                          gagaCGGACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAGGa        >  1:499276/1‑81 (MQ=255)                                                                gACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAGGACAaggc  <  2:499246/81‑1 (MQ=255)                                                                gACTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAGGACAagg   >  2:499280/1‑80 (MQ=255)                                                                 aCTAAC‑TTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAGGACAagg   >  1:499275/1‑79 (MQ=255)                                                                       |                                                                           ATCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAGGACAAGGC  >  NC_000913/2771806‑2771950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑