Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,849,928	Δ1 bp	intergenic (‑79/+46)	hycB ← / ← hycA	formate hydrogenlyase subunit HycB/regulator of the transcriptional regulator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,849,926	0	C	.	100.0%	155.0 / NA	37	intergenic (‑77/+48)	hycB/hycA	formate hydrogenlyase subunit HycB/regulator of the transcriptional regulator FhlA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/17);  total (20/17)

AGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTAC  >  NC_000913/2849851‑2850000                                                                            |                                                                           aGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGccatcc                                                                       >  1:515750/1‑77 (MQ=255)     aTTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCa                                                                   >  2:515745/1‑80 (MQ=255)        ccTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAAc                                                                <  1:515699/80‑1 (MQ=255)        ccTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAAc                                                                <  1:515698/80‑1 (MQ=255)         cTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAg                                                              <  1:515700/81‑1 (MQ=255)         cTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAg                                                              <  1:515701/81‑1 (MQ=255)         cTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAg                                                              <  1:515702/81‑1 (MQ=255)           cAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGc                                                            >  1:515758/1‑81 (MQ=255)               tGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTaa                                                        <  1:515703/81‑1 (MQ=255)                gATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAAc                                                       >  2:515761/1‑81 (MQ=255)                 aTTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACg                                                      <  1:515704/81‑1 (MQ=255)                 aTTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACg                                                      <  1:515705/81‑1 (MQ=255)                     tCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTg                                                  <  2:515706/81‑1 (MQ=255)                       aTTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGc                                                 >  1:515754/1‑80 (MQ=255)                       aTTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGc                                                 >  1:515737/1‑80 (MQ=255)                           ttGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAAc                                            >  1:515744/1‑81 (MQ=255)                            tGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACg                                           >  1:515752/1‑81 (MQ=255)                            tGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACg                                           >  2:515768/1‑81 (MQ=255)                              aCGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACggg                                         <  2:515707/81‑1 (MQ=255)                                     cATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGcc                                  >  1:515749/1‑81 (MQ=255)                                     cATGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGcc                                  <  2:515708/81‑1 (MQ=255)                                      aTGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCt                                 >  2:515751/1‑81 (MQ=255)                                      aTGCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCt                                 <  2:515709/81‑1 (MQ=255)                                        gCCGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCt                                 >  1:515760/1‑79 (MQ=255)                                         ccGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCa                               <  1:515710/80‑1 (MQ=255)                                          cGATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGc                             <  2:515711/81‑1 (MQ=255)                                           gATGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCt                            <  1:515712/81‑1 (MQ=255)                                            aTGAAATGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCt                            >  1:515743/1‑80 (MQ=255)                                                 aTGTCATTTTCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCAtgct                      >  1:515770/1‑81 (MQ=255)                                                         ttCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCt              >  2:515773/1‑81 (MQ=255)                                                          tCGACACTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCt              <  2:515713/80‑1 (MQ=255)                                                               acTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTcc        >  1:515775/1‑81 (MQ=255)                                                               acTCATCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTcc        >  2:515753/1‑81 (MQ=255)                                                                   aTCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCttt    <  2:515714/81‑1 (MQ=255)                                                                    tCGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTa   >  2:515771/1‑81 (MQ=255)                                                                     cGACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTAc  >  2:515755/1‑81 (MQ=255)                                                                      gACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTAc  >  1:515764/1‑80 (MQ=255)                                                                      gACACG‑CCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTAc  >  1:515756/1‑80 (MQ=255)                                                                            |                                                                           AGGCATTCCTCAGGTGATTGTCATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTAC  >  NC_000913/2849851‑2850000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑