Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,962,983	(T)5→4	coding (174/408 nt)	ygdB ←	DUF2509 domain‑containing protein YgdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,962,979	0	T	.	100.0%	142.7 / NA	34	coding (178/408 nt)	ygdB	DUF2509 domain‑containing protein YgdB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/14);  total (20/14)

CAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAG  >  NC_000913/2962899‑2963052                                                                                 |                                                                          cAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAt                                                                           >  2:540376/1‑81 (MQ=255) cAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAt                                                                           <  2:540313/81‑1 (MQ=255)      aaCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAttttcc                                                                      >  2:540377/1‑79 (MQ=255)          caaacaaaCCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCAc                                                                 >  2:540364/1‑81 (MQ=255)               aaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAg                                                            >  2:540388/1‑81 (MQ=255)                aacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAgc                                                           >  1:540367/1‑81 (MQ=255)                aacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAgc                                                           <  2:540314/81‑1 (MQ=255)                     gggCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGa                                                      >  2:540341/1‑81 (MQ=255)                      ggCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCg                                                       <  2:540315/79‑1 (MQ=255)                        cATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTg                                                   <  2:540316/81‑1 (MQ=255)                            ggTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAAc                                                >  1:540392/1‑80 (MQ=255)                             gTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGa                                              <  1:540317/81‑1 (MQ=255)                              tttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGAt                                             >  1:540371/1‑81 (MQ=255)                               ttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATg                                            >  2:540390/1‑81 (MQ=255)                               ttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATg                                            >  1:540386/1‑81 (MQ=255)                                 cAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGc                                          >  1:540363/1‑81 (MQ=255)                                   gCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCt                                        <  1:540318/81‑1 (MQ=255)                                   gCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCt                                        <  1:540319/81‑1 (MQ=255)                                    cGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTg                                       <  1:540320/81‑1 (MQ=255)                                     gTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg                                      >  2:540393/1‑81 (MQ=255)                                     gTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg                                      >  1:540385/1‑81 (MQ=255)                                     gTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg                                      >  1:540378/1‑81 (MQ=255)                                           cGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGgcggcg                                 <  2:540321/80‑1 (MQ=255)                                           cGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGgcggcg                                 >  2:540396/1‑80 (MQ=255)                                           cGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGgcgc                                <  2:540322/81‑1 (MQ=255)                                                aCTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtga                           >  2:540389/1‑81 (MQ=255)                                                    aaCTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgact                         <  2:540323/79‑1 (MQ=255)                                                         ctggctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACttt                   <  2:540324/80‑1 (MQ=255)                                                          tggctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTc                  >  1:540399/1‑80 (MQ=255)                                                           ggctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCa                >  2:540405/1‑81 (MQ=255)                                                            gctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGCACGATGGCCTGGCGGCGCAATGACTGACTTTCCAt               >  1:540381/1‑81 (MQ=255)                                                              tgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGc             >  1:540403/1‑81 (MQ=255)                                                               gCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCt            >  1:540401/1‑81 (MQ=255)                                                                cGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTc           <  2:540325/81‑1 (MQ=255)                                                                      ccAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcgc       >  2:540395/1‑79 (MQ=255)                                                                       cagcagTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCgag    <  2:540326/81‑1 (MQ=255)                                                                         gcagTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGagag  <  1:540327/81‑1 (MQ=255)                                                                                 |                                                                          CAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAG  >  NC_000913/2962899‑2963052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑