Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,967,332	Δ1 bp	coding (1103/2247 nt)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,967,331	0	G	.	100.0%	180.6 / NA	43	coding (1104/2247 nt)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (22/21);  total (22/21)

GCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACT  >  NC_000913/2967255‑2967406                                                                             |                                                                            gCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACgtagg                                                                         <  1:541370/81‑5 (MQ=255)       cACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATccc                                                                  >  2:541441/1‑81 (MQ=255)          cgcgATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTTGGGATCCTCAg                                                               >  2:541437/1‑81 (MQ=255)          cgcgATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAg                                                               >  1:541428/1‑81 (MQ=255)             gATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAgcg                                                             >  1:541430/1‑80 (MQ=255)              aTAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCa                                                           >  1:541424/1‑81 (MQ=255)               tAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCAc                                                          <  1:541371/81‑1 (MQ=255)                 gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACg                                                         <  1:541372/80‑1 (MQ=255)                 gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGt                                                        <  1:541373/81‑1 (MQ=255)                  ccATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTa                                                       <  1:541374/81‑1 (MQ=255)                   cATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTAc                                                      >  1:541429/1‑81 (MQ=255)                       aaCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCAt                                                   <  2:541375/80‑1 (MQ=255)                       aaCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCAt                                                   >  1:541455/1‑80 (MQ=255)                       aaCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCAt                                                   >  1:541405/1‑80 (MQ=255)                        aCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGa                                                 <  1:541376/81‑1 (MQ=255)                             cAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCg                                              >  2:541447/1‑79 (MQ=255)                              aGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCgcag                                           >  2:541444/1‑81 (MQ=255)                                 gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCAt                                        >  2:541467/1‑81 (MQ=255)                                  tCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATg                                       <  1:541377/81‑1 (MQ=255)                                    cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATggg                                     <  2:541378/81‑1 (MQ=255)                                    cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATgg                                      >  1:541439/1‑80 (MQ=255)                                       cGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGt                                  >  2:541462/1‑81 (MQ=255)                                        gATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGtt                                 <  2:541379/81‑1 (MQ=255)                                         aTGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTg                                <  2:541380/81‑1 (MQ=255)                                           gcagaaGCGAAGGCTGAATATCCGCGAGCAGCAC‑GTAGGGATACCCAGAGAACGTAACATGATCGAAGAATGGGAGTTGGc                              <  2:541385/81‑1 (MQ=255)                                            cagcaCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGc                              <  1:541382/80‑1 (MQ=255)                                            cagcaCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCt                             <  2:541381/81‑1 (MQ=255)                                            cagcaCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCt                             <  1:541383/81‑1 (MQ=255)                                               caCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTgcg                          <  2:541384/81‑1 (MQ=255)                                                   gAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCAt                      >  2:541450/1‑81 (MQ=255)                                                      ggCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCAtctc                   <  1:541386/81‑1 (MQ=255)                                                         tGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCa                >  1:541456/1‑81 (MQ=255)                                                           aaTATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCAc               <  1:541387/80‑1 (MQ=255)                                                             tatCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac            <  2:541388/81‑1 (MQ=255)                                                              atCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac            >  1:541446/1‑80 (MQ=255)                                                                 cgcgCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGacaaca        >  1:541443/1‑81 (MQ=255)                                                                 cgcgCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGacaaca        >  2:541448/1‑81 (MQ=255)                                                                  gcgcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAacac       >  2:541463/1‑81 (MQ=255)                                                                   cgcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAcc      <  2:541389/81‑1 (MQ=255)                                                                    gcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAcc      >  2:671095/1‑80 (MQ=255)                                                                    gcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCg     <  2:541391/81‑1 (MQ=255)                                                                    gcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCg     <  1:541390/81‑1 (MQ=255)                                                                     cccATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGa    >  2:541438/1‑81 (MQ=255)                                                                        aTCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACt  >  2:541432/1‑80 (MQ=255)                                                                             |                                                                            GCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACT  >  NC_000913/2967255‑2967406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑