Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	314,734	Δ1 bp	pseudogene (378/888 nt)	eaeH →	putative porin domain‑containing protein EaeH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	314,732	0	A	.	100.0%	93.9 / NA	23	pseudogene (376/888 nt)	eaeH	putative porin domain‑containing protein EaeH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/12);  total (11/12)

TGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTT  >  NC_000913/314652‑314805                                                                                 |                                                                          tGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATa                                                                           <  1:72362/81‑1 (MQ=255)             aagctaaCCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCt                                                              <  1:72363/81‑1 (MQ=255)               gctaaCCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTg                                                             >  2:72401/1‑80 (MQ=255)                ctaaCCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGaa                                                           <  2:72364/81‑1 (MQ=255)                        gAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGAttctt                                                   <  1:72365/81‑1 (MQ=255)                         aaaTACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGAttcttc                                                  <  1:72366/81‑1 (MQ=255)                           aTACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGc                                                >  2:72397/1‑81 (MQ=255)                              cAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTgg                                             <  1:72367/81‑1 (MQ=255)                              cAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTg                                              <  1:72369/80‑1 (MQ=255)                              cAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTg                                              <  2:72368/80‑1 (MQ=255)                                  aGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAAt                                         >  2:72402/1‑81 (MQ=255)                                        tCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTa                                   >  2:72407/1‑81 (MQ=255)                                          gggAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTAt                                  <  1:72370/80‑1 (MQ=255)                                          gggAAATATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATc                                 >  2:72396/1‑81 (MQ=255)                                             aaaTATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGa                              <  2:72371/81‑1 (MQ=255)                                             aaaTATGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGa                              >  1:72408/1‑81 (MQ=255)                                                 atGGTACTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGCTTCTTCGCTGGAAATGCTTTATCCGATTTa                          >  2:72400/1‑81 (MQ=255)                                                    gTACTGCGCGCGTCAAACTGAAAGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGa                       >  2:72405/1‑81 (MQ=255)                                                       cTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATAc                    <  2:419074/81‑1 (MQ=255)                                                       cTGCGCGCGTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATAc                    >  2:72413/1‑81 (MQ=255)                                                          cgcgcgTCAAACTGAATGTCGAT‑AAGATTTCACGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGcc                 <  2:72372/81‑1 (MQ=255)                                                             gcgTCAAACTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGa               >  2:72399/1‑80 (MQ=255)                                                                     cTGAATGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATAtgt      <  1:72373/81‑1 (MQ=255)                                                                          tGTCGAT‑AAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATgttgtt  >  2:72409/1‑80 (MQ=255)                                                                                 |                                                                          TGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTT  >  NC_000913/314652‑314805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑