Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,119,442	(A)7→6	intergenic (‑330/+155)	yghJ ← / ← glcA	putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,119,436	0	A	.	100.0%	49.8 / NA	13	intergenic (‑324/+161)	yghJ/glcA	putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/4);  total (9/4)

GCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCAT  >  NC_000913/3119365‑3119505                                                                        |                                                                      gCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaaatag                                                               <  1:575551/80‑4 (MQ=255)   aaaaGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATg                                                           >  1:575565/1‑81 (MQ=255)       gCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTtct                                                       <  1:575552/81‑1 (MQ=255)          aCAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCAc                                                    >  2:575571/1‑81 (MQ=255)                   aCGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAat                                           >  1:575570/1‑81 (MQ=255)                           tGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAg                                    >  1:575579/1‑80 (MQ=255)                                   aaGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATc                           <  2:575553/81‑1 (MQ=255)                                   aaGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATc                           >  2:575568/1‑81 (MQ=255)                                         aCATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACg                     <  1:575555/81‑1 (MQ=255)                                            taattaatACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCa                   >  2:575574/1‑80 (MQ=255)                                             aattaatACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCaa                  >  2:575566/1‑80 (MQ=255)                                                  atACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACat             <  2:575556/80‑1 (MQ=255)                                                    aCAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACattat          >  1:575572/1‑81 (MQ=255)                                                            gTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCAt  >  2:575560/1‑81 (MQ=255)                                                                        |                                                                      GCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCAT  >  NC_000913/3119365‑3119505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑