Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,358	(A)7→6	coding (228/693 nt)	yghT →	putative ATP‑binding protein YghT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,352	0	A	.	100.0%	106.9 / NA	26	coding (222/693 nt)	yghT	putative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (14/12);  total (14/12)

CGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATA  >  NC_000913/3134274‑3134428                                                                               |                                                                             cggtcgTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCaa                                                                             >  2:579263/1‑80 (MQ=255)             gACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGa                                                               >  1:579255/1‑81 (MQ=255)                   aTTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGtt                                                         >  1:579257/1‑81 (MQ=255)                    ttACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTc                                                        >  1:579259/1‑81 (MQ=255)                     tACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTcc                                                       >  2:579256/1‑81 (MQ=255)                     tACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTcc                                                       >  1:579253/1‑81 (MQ=255)                      aCCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTcc                                                       <  2:579216/80‑1 (MQ=255)                       ccGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCg                                                      <  1:579217/80‑1 (MQ=255)                           tGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCt                                                 >  2:579250/1‑81 (MQ=255)                             aTGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTgg                                               <  1:579218/81‑1 (MQ=255)                               gggAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGt                                              >  1:579267/1‑80 (MQ=255)                                 gAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAAt                                           >  1:579266/1‑81 (MQ=255)                                    aaTCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTAc                                        <  1:579219/81‑1 (MQ=255)                                      tCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAg                                      >  1:579226/1‑81 (MQ=255)                                         ttACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGt                                   >  2:579272/1‑81 (MQ=255)                                           aCATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGtt                                  <  1:579220/80‑1 (MQ=255)                                              tAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTc                              <  2:579221/81‑1 (MQ=255)                                                        gAACGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCt                    >  2:579258/1‑81 (MQ=255)                                                         aaCGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCt                    <  1:579222/80‑1 (MQ=255)                                                           cGAAATCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTAc                 <  1:579223/81‑1 (MQ=255)                                                               aTCAGGTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTgcg             <  2:579224/81‑1 (MQ=255)                                                                   ggTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCttt         <  1:579225/81‑1 (MQ=255)                                                                    gTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTc        >  1:579261/1‑81 (MQ=255)                                                                    gTGAAAACAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTc        <  2:579226/81‑1 (MQ=255)                                                                        aaaCAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCa    >  2:579303/1‑81 (MQ=255)                                                                         aaCAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCat   <  2:579227/81‑1 (MQ=255)                                                                          aCAGC‑AAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCata  >  2:579264/1‑81 (MQ=255)                                                                               |                                                                             CGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATA  >  NC_000913/3134274‑3134428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑