Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,302,518	Δ1 bp	coding (30/879 nt)	yhbV →	putative peptidase YhbV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,302,517	0	G	.	100.0%	155.0 / NA	37	coding (29/879 nt)	yhbV	putative peptidase YhbV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/14);  total (23/14)

CGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGT  >  NC_000913/3302440‑3302593                                                                              |                                                                             cGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCtgtgtac                                                                           <  1:619601/81‑4 (MQ=255)   cAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTg                                                                        <  2:619602/81‑1 (MQ=255)      aCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGc                                                                      >  1:619637/1‑80 (MQ=255)       ccACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCa                                                                    <  1:619603/81‑1 (MQ=255)          ctctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCaa                                                                   >  1:619644/1‑79 (MQ=255)              tGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAgaga                                                             >  2:619640/1‑81 (MQ=255)              tGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAgaga                                                             >  2:619659/1‑81 (MQ=255)                gcgcATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACg                                                           >  2:619664/1‑81 (MQ=255)                gcgcATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACg                                                           >  2:619639/1‑81 (MQ=255)                gcgcATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACg                                                           >  2:619662/1‑81 (MQ=255)                  gcATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCt                                                         >  1:619638/1‑81 (MQ=255)                      atCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTgg                                                       >  2:619641/1‑79 (MQ=255)                       tCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGgaag                                                    <  1:619604/81‑1 (MQ=255)                              aaaTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTa                                             >  1:619650/1‑81 (MQ=255)                                aTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATc                                           <  1:619605/81‑1 (MQ=255)                                aTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATc                                           <  2:619606/81‑1 (MQ=255)                                 tGGCAGTGAGAAAAGCAATGAAATATTCCTTTGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTAt                                            >  1:619636/1‑79 (MQ=255)                                      gTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAgg                                     <  1:619607/81‑1 (MQ=255)                                        gagaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGgcc                                   >  2:619651/1‑81 (MQ=255)                                        gagaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGgcc                                   >  1:619657/1‑81 (MQ=255)                                           aaaaGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGgccgc                                 >  2:619656/1‑80 (MQ=255)                                            aaaGCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGcca                               <  2:619608/81‑1 (MQ=255)                                               gCAATGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGccac                              >  1:619703/1‑79 (MQ=255)                                                 aaTGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACcag                           >  1:619642/1‑80 (MQ=255)                                                   tGAAATATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACcagcag                        >  2:619646/1‑81 (MQ=255)                                                     aaaTATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAgcg                      >  2:619660/1‑81 (MQ=255)                                                     aaaTATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAgcg                      <  1:619609/81‑1 (MQ=255)                                                      aaTATTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAgcgc                     >  1:619653/1‑81 (MQ=255)                                                       atatTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGcc                    <  2:619610/81‑1 (MQ=255)                                                       atatTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGcc                    <  2:619612/81‑1 (MQ=255)                                                       atatTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGcc                    <  1:619613/81‑1 (MQ=255)                                                       atatTCCTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGcc                    <  1:619611/81‑1 (MQ=255)                                                            ccTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGt               >  1:619649/1‑81 (MQ=255)                                                            ccTTAGGGCCAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGt               >  2:619645/1‑81 (MQ=255)                                                                    ccAGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATc       <  1:619614/81‑1 (MQ=255)                                                                      aGTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCtt     <  2:619615/81‑1 (MQ=255)                                                                       gTGCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTg    >  2:619658/1‑81 (MQ=255)                                                                         gCTGT‑GTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGt  >  2:619648/1‑81 (MQ=255)                                                                              |                                                                             CGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGT  >  NC_000913/3302440‑3302593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑