Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	346,601	(A)6→5	coding (118/276 nt)	yahO →	DUF1471 domain‑containing protein YahO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	346,596	0	A	.	100.0%	96.8 / NA	24	coding (113/276 nt)	yahO	DUF1471 domain‑containing protein YahO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (14/10);  total (14/10)

TAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGC  >  NC_000913/346518‑346668                                                                               |                                                                         tagcgttagcCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGaa                                                                         >  1:78652/1‑80 (MQ=255)   gcgttagcCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGaaaaa                                                                      >  2:78645/1‑81 (MQ=255)          ccGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGa                                                              >  2:78647/1‑81 (MQ=255)                  aaTGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCaa                                                      >  2:78649/1‑81 (MQ=255)                   aTGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAAc                                                     <  2:78630/81‑1 (MQ=255)                        tATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGc                                                 >  1:78656/1‑80 (MQ=255)                         aTGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCaa                                               >  1:78641/1‑81 (MQ=255)                              gCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGaaa                                          <  1:78631/81‑1 (MQ=255)                                      gatgaCCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACt                                  >  2:78664/1‑81 (MQ=255)                                       atgaCCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTg                                 >  1:78667/1‑81 (MQ=255)                                          aCCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTg                                 >  2:78672/1‑78 (MQ=255)                                          aCCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTg                                 >  2:78662/1‑78 (MQ=255)                                            cAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGAt                            <  2:78632/81‑1 (MQ=255)                                               aGCGGAATTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCa                         <  1:78633/81‑1 (MQ=255)                                                     aTTTGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAaagaa                   <  2:78634/81‑1 (MQ=255)                                                      tttGAAAAAGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAaagaag                  <  2:78635/81‑1 (MQ=255)                                                           aaaaGTTGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGAttt              >  1:78659/1‑80 (MQ=255)                                                                ttGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCaa        <  1:78636/81‑1 (MQ=255)                                                                 tGAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCaa        >  1:78651/1‑80 (MQ=255)                                                                  gAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCaaa       <  2:78637/80‑1 (MQ=255)                                                                  gAATCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCaaa       >  2:78661/1‑80 (MQ=255)                                                                   aaTCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCaaaa      >  1:78665/1‑80 (MQ=255)                                                                    aTCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCaaaaaa    <  2:78638/81‑1 (MQ=255)                                                                    aTCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCaaaa      >  2:78653/1‑79 (MQ=255)                                                                     tCGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAg   <  1:78639/81‑1 (MQ=255)                                                                      cGCAGTATG‑AAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGc  >  2:78650/1‑81 (MQ=255)                                                                               |                                                                         TAGCGTTAGCCGTTACCAATGTCTATGCCGCTGAATTGATGACCAAAGCGGAATTTGAAAAAGTTGAATCGCAGTATGAAAAAATAGGTGATATTTCAACCAGCAATGAAATGTCGACTGCAGATGCAAAAGAAGATTTGATCAAAAAAGC  >  NC_000913/346518‑346668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑