| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,569,344 | (T)6→5 | intergenic (‑16/+2) | glgC ← / ← glgX | glucose‑1‑phosphate adenylyltransferase/limit dextrin alpha‑1,6‑glucohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,569,339 | 0 | T | . | 97.1% | 134.6 / ‑1.5 | 36 | intergenic (‑11/+7) | glgC/glgX | glucose‑1‑phosphate adenylyltransferase/limit dextrin alpha‑1,6‑glucohydrolase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (20/14); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCT > NC_000913/3569260‑3569415 | cAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCtt > 2:687941/1‑81 (MQ=255) aGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCttt < 2:687884/81‑1 (MQ=255) aGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCtt < 2:687883/80‑1 (MQ=255) aGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCtt < 1:687882/80‑1 (MQ=255) gggCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCtttt < 1:687885/81‑1 (MQ=255) gggCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCtttt > 2:687939/1‑81 (MQ=255) ggCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCttttt < 1:687886/81‑1 (MQ=255) gCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCttttta < 2:687887/81‑2 (MQ=255) cAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTatcat > 2:687961/1‑81 (MQ=255) gATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCAtct < 1:687888/81‑1 (MQ=255) tttCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATctct < 1:687889/81‑1 (MQ=255) aTGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGaa > 2:687959/1‑80 (MQ=255) aTGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAac < 1:687891/81‑1 (MQ=255) aTGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAac < 2:687890/81‑1 (MQ=255) gccgCTGGCGCGCCAACCTTAAGTGATCGTTCTTCTCTAAACTAACCATGACTCACTCC‑TTTTAAACATCTCTGGAacaca < 2:687893/78‑1 (MQ=255) cAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAacacac < 2:687892/81‑1 (MQ=255) cgcgcCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATcc < 1:687894/81‑1 (MQ=255) gcCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCgtg > 1:687943/1‑81 (MQ=255) ccAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCgtgt > 1:687934/1‑81 (MQ=255) tAAGTAATCGTTCTTCTCAAAACTAACAAT‑ACCAAC‑CCCCTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTc < 1:687895/80‑1 (MQ=255) aaGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTcc > 2:687942/1‑81 (MQ=255) aGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTccc > 2:688013/1‑81 (MQ=255) aGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTccc > 1:687938/1‑81 (MQ=255) gTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCt < 1:687896/81‑1 (MQ=255) gTGATCGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCt > 1:687953/1‑81 (MQ=255) gATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTg > 1:687962/1‑81 (MQ=255) cGTTCTTCTCTAAACTAACCATGACTAACTCC‑TTTCTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGc > 1:687940/1‑78 (MQ=255) ttcttcTCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGAc < 1:687897/81‑1 (MQ=255) ttctCTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGc > 1:687915/1‑81 (MQ=255) tctcTAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGcc > 1:687947/1‑81 (MQ=255) tAAACTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTaa > 1:687944/1‑81 (MQ=255) aaaCTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGAGCAGGTCCCTGCCAGACAGCCGTAAt > 2:687950/1‑81 (MQ=255) aaCTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACTCACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTa < 2:687898/78‑1 (MQ=255) cTAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCAc > 1:687968/1‑81 (MQ=255) tAACCATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACt > 1:687952/1‑81 (MQ=255) ccATGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTggg < 2:687899/81‑1 (MQ=255) aTGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGtt < 2:687900/81‑1 (MQ=255) aTGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGtt < 2:687901/81‑1 (MQ=255) aTGACTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGt > 2:687956/1‑80 (MQ=255) cTAACTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATcc > 2:687966/1‑81 (MQ=255) aaCTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCctc > 2:688002/1‑81 (MQ=255) aCTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCctct > 1:687977/1‑81 (MQ=255) aCTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCctct > 2:687969/1‑81 (MQ=255) aCTCC‑TTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCct < 1:687902/79‑1 (MQ=255) | CAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCT > NC_000913/3569260‑3569415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |