Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,672,287	Δ1 bp	intergenic (‑393/‑127)	yhjB ← / → yhjC	putative DNA‑binding transcriptional regulator YhjB/putative DNA‑binding transcriptional regulator YhjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,672,284	0	T	.	100.0%	111.2 / NA	27	intergenic (‑390/‑130)	yhjB/yhjC	putative DNA‑binding transcriptional regulator YhjB/putative DNA‑binding transcriptional regulator YhjC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (15/12);  total (15/12)

CTCTGTTCACTTTAAAGGGAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCCTTTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCATTAGATGTTAGAAAAAA  >  NC_000913/3672204‑3672354                                                                                 |                                                                       ctctGTTCACTTTAAAGGGAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCCt                                                                        <  1:713776/81‑1 (MQ=255)         aCTTTAAAGGGAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCctgc                                                                <  2:713777/80‑1 (MQ=255)           tttAAAGGGAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCctgct                                                               >  1:713795/1‑79 (MQ=255)             tAAAGGGAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTgag                                                            <  1:713778/80‑1 (MQ=255)              aaaGGGAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTgaga                                                           >  1:713828/1‑80 (MQ=255)               aaGGGAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGaaa                                                         >  1:713834/1‑81 (MQ=255)                  ggAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCg                                                      >  2:713827/1‑81 (MQ=255)                  ggAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCg                                                      <  2:713779/81‑1 (MQ=255)                       cGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCcaca                                                 >  1:713836/1‑81 (MQ=255)                         cTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACAtt                                               >  1:713829/1‑81 (MQ=255)                                          tttAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACAtt                                <  1:713780/79‑1 (MQ=255)                                          tttAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACAt                                 <  2:713781/78‑1 (MQ=255)                                          tttAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATtgt                              >  1:713825/1‑81 (MQ=255)                                           ttAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATtgt                              >  1:713821/1‑80 (MQ=255)                                            tAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATtgtg                             >  1:713837/1‑80 (MQ=255)                                             aaGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGa                            <  1:713782/80‑1 (MQ=255)                                                 ggATTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAAccc                       >  1:713839/1‑81 (MQ=255)                                                   aTTCACCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCgg                     <  2:713783/81‑1 (MQ=255)                                                       aCCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCAtt                 <  2:713785/81‑1 (MQ=255)                                                       aCCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCAt                  >  1:713840/1‑80 (MQ=255)                                                       aCCTTTCTCAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCAt                  <  1:713784/80‑1 (MQ=255)                                                            tctcAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCAGGCATTAGATg            >  2:713841/1‑81 (MQ=255)                                                               cAGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCATTAGATGt           >  2:713851/1‑79 (MQ=255)                                                                aGAAAGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCATTAGATGtt          <  1:713786/79‑1 (MQ=255)                                                                  aaaGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCATTAGATGTTAGaa      <  2:713787/81‑1 (MQ=255)                                                                   aaGCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCATTAGATGTTAGaaa     <  1:713788/81‑1 (MQ=255)                                                                     gCTATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCATTAGATGTTAGaaaa    >  1:713850/1‑80 (MQ=255)                                                                       tATTCCGCCC‑TTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCATTAGATGTTAGaaaaaa  >  1:713845/1‑80 (MQ=255)                                                                                 |                                                                       CTCTGTTCACTTTAAAGGGAATCGCTGAAAAATACGCTCTGTTTAAGGGGATTCACCTTTCTCAGAAAGCTATTCCGCCCTTTTCCTGCTGAGAAATCGCCACATTCGGCATGACAACATTGTGAAACCCGGCATTAGATGTTAGAAAAAA  >  NC_000913/3672204‑3672354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑