Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,710,393	(A)6→5	coding (83/1692 nt)	eptB ←	Kdo2‑lipid A phosphoethanolamine 7''‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,710,388	0	A	.	100.0%	128.5 / NA	31	coding (88/1692 nt)	eptB	Kdo2‑lipid A phosphoethanolamine 7''‑transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/20);  total (11/20)

ACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGTAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTG  >  NC_000913/3710309‑3710464                                                                                |                                                                             aCGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGTa                                                                              >  2:724351/1‑80 (MQ=255)  cGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGTaa                                                                             >  1:724346/1‑80 (MQ=255)     tGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGTaaaaac                                                                         <  1:724298/81‑2 (MQ=255)       gcggcCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGTaaaaacc                                                                        <  1:724299/80‑3 (MQ=255)       gcggcCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGTaaaaacc                                                                        <  1:724300/80‑3 (MQ=255)       gcggcCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGTaaaaacc                                                                        <  2:724301/80‑3 (MQ=255)          gcCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCgcgc                                                                   >  1:724343/1‑81 (MQ=255)           ccAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGcc                                                                  <  2:724302/81‑1 (MQ=255)            cAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCa                                                                 >  2:724349/1‑81 (MQ=255)              gTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCAtt                                                               <  2:724304/81‑1 (MQ=255)              gTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCAtt                                                               <  1:724303/81‑1 (MQ=255)               ttCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTc                                                              <  2:724305/81‑1 (MQ=255)                  aacaacAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATa                                                           <  2:724306/81‑1 (MQ=255)                       cagcagAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAg                                                      <  1:724307/81‑1 (MQ=255)                           agAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCa                                                  <  2:724308/81‑1 (MQ=255)                            gAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCaa                                                 <  1:724309/81‑1 (MQ=255)                                 gCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAata                                               >  1:724353/1‑78 (MQ=255)                                  ccTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGa                                           <  1:724310/81‑1 (MQ=255)                                       ccAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTgc                                       <  2:724311/80‑1 (MQ=255)                                           aCGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTgcaagca                                  <  1:724312/81‑1 (MQ=255)                                            cGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTgcaagcaa                                 <  2:724313/81‑1 (MQ=255)                                             ggTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCaaa                                <  2:724314/81‑1 (MQ=255)                                               tAAAATCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCaaaaa                              <  1:724315/81‑1 (MQ=255)                                                 aaaTCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAg                             <  2:724316/80‑1 (MQ=255)                                                 aaaTCGTGCGCATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGc                            >  2:724352/1‑81 (MQ=255)                                                        gcgcATAGCTGCCGAAGCGGCGGT‑AAAAACTGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCtt                     >  2:724377/1‑81 (MQ=255)                                                        gcgcATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCtt                     >  2:724360/1‑81 (MQ=255)                                                        gcgcATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCtt                     >  2:724370/1‑81 (MQ=255)                                                          gcATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTct                   >  2:724350/1‑81 (MQ=255)                                                           cATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTctg                  >  2:724347/1‑81 (MQ=255)                                                           cATAGCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTctg                  <  1:724317/81‑1 (MQ=255)                                                               gCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCtgt              <  2:724319/81‑1 (MQ=255)                                                               gCTGCCGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCtgt              <  1:724318/81‑1 (MQ=255)                                                                   ccGAAGCGGCGGT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTgtgt            <  1:724320/79‑1 (MQ=255)                                                                         cggcggT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGAtt    >  2:724361/1‑81 (MQ=255)                                                                         cggcggT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGAtt    >  2:724362/1‑81 (MQ=255)                                                                           gcggT‑AAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTg  <  1:724321/81‑1 (MQ=255)                                                                                |                                                                             ACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCTGCCGAAGCGGCGGTAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTG  >  NC_000913/3710309‑3710464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑