Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,710,634	Δ1 bp	intergenic (‑159/+165)	eptB ← / ← yhjX	Kdo2‑lipid A phosphoethanolamine 7''‑transferase/putative pyruvate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,710,633	0	A	.	100.0%	107.3 / NA	26	intergenic (‑158/+166)	eptB/yhjX	Kdo2‑lipid A phosphoethanolamine 7''‑transferase/putative pyruvate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/13);  total (13/13)

TAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATT  >  NC_000913/3710554‑3710709                                                                                |                                                                             tAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGag                                                                             >  2:724383/1‑80 (MQ=255)  aGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGag                                                                             >  2:724393/1‑79 (MQ=255)     cAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGagcac                                                                          >  2:724388/1‑76 (MQ=255)       gATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACaaa                                                                      >  2:724386/1‑81 (MQ=255)       gATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACaaa                                                                      <  2:724353/81‑1 (MQ=255)        aTAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAAt                                                                     >  1:724406/1‑81 (MQ=255)                aaTCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACaaa                                                              >  2:724389/1‑80 (MQ=255)                      cacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGc                                                         <  1:724354/79‑1 (MQ=255)                           gcgAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTg                                                  <  2:724355/81‑1 (MQ=255)                                  aGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGaaaa                                              >  2:724399/1‑78 (MQ=255)                                  aGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAagag                                           <  2:724356/81‑1 (MQ=255)                                     aGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACt                                        >  2:724390/1‑81 (MQ=255)                                       aCCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACttt                                      <  2:724357/81‑1 (MQ=255)                                         cacaGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAg                                    >  2:724392/1‑81 (MQ=255)                                            aGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGt                                 <  1:724358/81‑1 (MQ=255)                                              cAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTa                               <  1:724359/81‑1 (MQ=255)                                               aaaGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTa                               >  2:724416/1‑80 (MQ=255)                                                aaGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACa                             <  1:724360/81‑1 (MQ=255)                                                  gggAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACaaa                           <  1:724361/81‑1 (MQ=255)                                                   ggAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACaaa                           >  1:724411/1‑80 (MQ=255)                                                     aaaaaGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAg                        <  1:724362/81‑1 (MQ=255)                                                         aGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAg                     >  2:724410/1‑80 (MQ=255)                                                                   aaGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGcc          <  2:724363/81‑1 (MQ=255)                                                                    agggtgtGCATG‑AGCACAAATATACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCa         <  2:724364/78‑1 (MQ=255)                                                                    agagTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGc           >  1:724405/1‑79 (MQ=255)                                                                     gagTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAg        <  1:724365/81‑1 (MQ=255)                                                                        tgtgCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGtt      >  1:724408/1‑80 (MQ=255)                                                                           gCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAAtt  >  1:724409/1‑81 (MQ=255)                                                                           gCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAAtt  >  2:724400/1‑81 (MQ=255)                                                                                |                                                                             TAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATT  >  NC_000913/3710554‑3710709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑