Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,743,122	(A)5→4	coding (390/999 nt)	yiaK →	2,3‑diketo‑L‑gulonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,743,118	0	A	.	100.0%	229.2 / NA	54	coding (386/999 nt)	yiaK	2,3‑diketo‑L‑gulonate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (33/21);  total (33/21)

GATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGAAAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAGTGTCGCAT  >  NC_000913/3743039‑3743193                                                                                |                                                                            gATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGa                                                                             >  2:732652/1‑80 (MQ=255)    cACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGaaaag                                                                         >  2:732663/1‑80 (MQ=255)     aCGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGaaaagg                                                                        >  2:732688/1‑79 (MQ=255)      cGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGaaaaggc                                                                       >  2:732665/1‑78 (MQ=255)       ggTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGaaaaggct                                                                      <  2:732599/81‑5 (MQ=255)       ggTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGaaaaggct                                                                      <  1:732598/81‑5 (MQ=255)           ttGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTatat                                                                 <  1:732600/81‑1 (MQ=255)             ggTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTg                                                               >  1:732682/1‑81 (MQ=255)              gTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTgg                                                              >  2:732710/1‑81 (MQ=255)               tCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGc                                                             <  2:732601/81‑1 (MQ=255)                cTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCa                                                            >  2:732692/1‑81 (MQ=255)                cTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCa                                                            >  2:732670/1‑81 (MQ=255)                cTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCa                                                            >  2:732711/1‑81 (MQ=255)                   gtggCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCa                                                            <  1:732602/78‑1 (MQ=255)                   gtggCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCAtt                                                          <  2:732604/80‑1 (MQ=255)                   gtggCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCAt                                                           <  1:732603/79‑1 (MQ=255)                        aCTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTg                                                     >  1:732699/1‑80 (MQ=255)                         cTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGa                                                   <  2:732605/81‑1 (MQ=255)                          tACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGAc                                                  >  1:732644/1‑81 (MQ=255)                           aCGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGAc                                                  >  1:732673/1‑80 (MQ=255)                              tAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAAc                                              <  1:732606/81‑1 (MQ=255)                               aaTGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACt                                             >  2:732690/1‑81 (MQ=255)                               aaTGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACt                                             >  2:732685/1‑81 (MQ=255)                                  gCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCa                                          >  1:732687/1‑81 (MQ=255)                                   ccAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCa                                          <  1:732607/80‑1 (MQ=255)                                    cAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATc                                        <  1:732608/81‑1 (MQ=255)                                     aaCCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATcc                                       >  2:732691/1‑80 (MQ=255)                                      aCCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AACAGGCTATATTGGCATTTGCTGGACCAACTCCATCGc                                      >  1:732721/1‑81 (MQ=255)                                       ccACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGcc                                     <  2:732609/81‑1 (MQ=255)                                         aCTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGt                                   <  2:732610/81‑1 (MQ=255)                                          cTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGt                                   >  2:732738/1‑80 (MQ=255)                                             gATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAAt                                <  1:732612/80‑1 (MQ=255)                                             gATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAAt                                <  1:732611/80‑1 (MQ=255)                                               tGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATgcc                             >  1:732704/1‑81 (MQ=255)                                                 cgcgGCGGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATgccgc                           >  1:732675/1‑81 (MQ=255)                                                  gcggcgGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTACTgccgcc                          >  2:732708/1‑81 (MQ=255)                                                  gcggcgGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATgccgcc                          <  2:732613/81‑1 (MQ=255)                                                  gcggcgGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATgccgcc                          >  2:732712/1‑81 (MQ=255)                                                  gcggcgGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATgccgcc                          >  1:732680/1‑81 (MQ=255)                                                  gcggcgGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATgccgcc                          <  1:732614/81‑1 (MQ=255)                                                  gcggcgGCAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATgccgcc                          <  1:732615/81‑1 (MQ=255)                                                    ggcggcAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGt                        >  2:732694/1‑81 (MQ=255)                                                    ggcggcAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGt                        >  2:732701/1‑81 (MQ=255)                                                    ggcggcAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGt                        >  2:732702/1‑81 (MQ=255)                                                      cggcAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTgg                      <  1:732616/81‑1 (MQ=255)                                                       ggcAGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTggg                     >  2:732720/1‑81 (MQ=255)                                                          aGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGgcg                  >  1:732681/1‑81 (MQ=255)                                                          aGCTACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGgcg                  >  2:732686/1‑81 (MQ=255)                                                             tACGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCa                >  2:732684/1‑80 (MQ=255)                                                              aCGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCaa               >  1:732700/1‑80 (MQ=255)                                                               cGGCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCACCGTGGGGCGCaa               >  2:732719/1‑79 (MQ=255)                                                                 gCTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCaaaa             >  2:732732/1‑79 (MQ=255)                                                                  cTGGCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAagag          >  2:732679/1‑81 (MQ=255)                                                                     gCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAgtgt       >  2:732731/1‑81 (MQ=255)                                                                     gCAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAgtgt       <  2:732617/81‑1 (MQ=255)                                                                      cAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAGTGTc      >  1:732709/1‑81 (MQ=255)                                                                      cAGGCGGCGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAGTGTc      <  1:732618/81‑1 (MQ=255)                                                                        ggcggcGG‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAGTGTCGc    <  1:732619/81‑1 (MQ=255)                                                                          cggcgg‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAGTGTCGCAt  <  2:732620/81‑1 (MQ=255)                                                                           ggcgg‑AAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAGTGTCGCa   >  1:732716/1‑79 (MQ=255)                                                                                |                                                                            GATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGAAAAAGGCTATATTGGCATTTGCTGGACCAACTCCATCGCCGTAATGCCGCCGTGGGGCGCAAAAGAGTGTCGCAT  >  NC_000913/3743039‑3743193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑