Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,797,159	(A)6→5	coding (212/1260 nt)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,154	0	A	.	100.0%	23.5 / NA	7	coding (207/1260 nt)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/3);  total (4/3)

AGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTT  >  NC_000913/3797074‑3797222                                                                                 |                                                                     aGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATtataatata                                                                      <  1:746987/81‑1 (MQ=255)             tataATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTAttc                                                         >  2:746996/1‑81 (MQ=255)              ataataTCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTAttct                                                        >  2:746999/1‑81 (MQ=255)                  tatCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTccc                                                    >  1:746997/1‑81 (MQ=255)                               gTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATAtttt                                       <  2:746988/81‑1 (MQ=255)                                                  ttttACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGAttt                    <  1:746989/81‑1 (MQ=255)                                                        gTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTg              >  2:747001/1‑81 (MQ=255)                                                                    aaaaTTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGtt  <  1:746990/81‑1 (MQ=255)                                                                                 |                                                                     AGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTT  >  NC_000913/3797074‑3797222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑