Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,819	(T)6→5	coding (181/1221 nt)	sbmA →	peptide antibiotic/peptide nucleic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,814	0	T	.	100.0%	85.1 / NA	21	coding (176/1221 nt)	sbmA	peptide antibiotic/peptide nucleic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/7);  total (14/7)

ACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGCATCG  >  NC_000913/396736‑396883                                                                               |                                                                      aCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttt                                                                     <  2:89679/81‑1 (MQ=255)      gTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAtttttacg                                                                >  1:89712/1‑78 (MQ=255)                tCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACAt                                                     <  1:89680/81‑1 (MQ=255)                tCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACAt                                                     >  1:89714/1‑81 (MQ=255)                  aCCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTg                                                   >  2:89699/1‑81 (MQ=255)                      gcgcTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTg                                               >  1:89713/1‑81 (MQ=255)                        gcTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCg                                             >  1:89723/1‑81 (MQ=255)                              gggCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGAc                                       >  2:89719/1‑81 (MQ=255)                                gCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTCTTACGCTTACTACATTGTTTGCGTAGGACtt                                     >  2:89711/1‑81 (MQ=255)                                 cAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACttt                                    >  2:89726/1‑81 (MQ=255)                                  aGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACtttt                                   >  2:89739/1‑81 (MQ=255)                                  aGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACtttt                                   >  2:89707/1‑81 (MQ=255)                                       ccGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCAt                              >  1:89709/1‑81 (MQ=255)                                           ttAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGtt                          <  2:89681/81‑1 (MQ=255)                                             aGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCt                        >  1:89717/1‑81 (MQ=255)                                                   gcgcgTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTa                  >  2:89718/1‑81 (MQ=255)                                                      cgTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATc                >  1:89720/1‑80 (MQ=255)                                                      cgTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATc                >  2:89728/1‑80 (MQ=255)                                                      cgTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCt               <  1:89682/81‑1 (MQ=255)                                                          tCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACa            <  1:89683/80‑1 (MQ=255)                                                           cTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAg           <  1:89684/80‑1 (MQ=255)                                                              gTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGcc         <  1:89685/79‑1 (MQ=255)                                                                    ggATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGCATCg  <  2:89686/80‑1 (MQ=255)                                                                               |                                                                      ACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGCATCG  >  NC_000913/396736‑396883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑