Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,060,925	Δ1 bp	coding (479/711 nt)	yihL →	putative transcriptional regulator YihL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,060,922	0	A	.	100.0%	111.3 / NA	27	coding (476/711 nt)	yihL	putative transcriptional regulator YihL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (16/11);  total (16/11)

TGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAA  >  NC_000913/4060847‑4060994                                                                            |                                                                         tGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGaaacca                                                                     >  2:818663/1‑78 (MQ=255)    aaGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACt                                                                  >  2:818672/1‑80 (MQ=255)      ggACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCAt                                                               >  1:818681/1‑81 (MQ=255)         cATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCAtttt                                                            <  1:818647/81‑1 (MQ=255)           tAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCt                                                          <  2:818648/81‑1 (MQ=255)             aaGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTg                                                         >  1:818679/1‑80 (MQ=255)              aGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTg                                                         >  2:818685/1‑79 (MQ=255)                  ttCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCt                                                   >  2:818683/1‑81 (MQ=255)                    cTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTg                                                  <  1:818649/80‑1 (MQ=255)                      aTCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTggg                                                >  1:818676/1‑80 (MQ=255)                       tCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTgg                                                 <  2:818650/78‑1 (MQ=255)                       tCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTgg                                                 <  2:818651/78‑1 (MQ=255)                            caaCATATATTAATCCTGAAGTTTCTCCTTGCTTTCTTGCCCCCCTTG‑AAACCACTCATTTTCTGCAGTCTGGGACACGTg                                         >  1:818678/2‑81 (MQ=255)                                atatatTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTAc                                     <  1:818652/81‑1 (MQ=255)                                   tatTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCa                                   >  1:818671/1‑80 (MQ=255)                                             gAAGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACgg                        >  2:818691/1‑81 (MQ=255)                                               aGTTGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACgg                        >  2:818680/1‑79 (MQ=255)                                                  tGCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGgtagt                    >  2:818693/1‑80 (MQ=255)                                                   gCTCCGGGCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGgtagta                   >  2:818686/1‑80 (MQ=255)                                                         ggCTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAAt            >  1:818687/1‑81 (MQ=255)                                                           cTTTATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTg          <  1:818653/81‑1 (MQ=255)                                                            tttATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGa         >  1:818699/1‑81 (MQ=255)                                                              tATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGAt        <  1:818655/80‑1 (MQ=255)                                                              tATTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGAt        <  1:818654/80‑1 (MQ=255)                                                               aTTGAACAACTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGAttt      >  2:818695/1‑81 (MQ=255)                                                                  gAACAACTTG‑AAACCACGCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTc    >  1:818690/1‑80 (MQ=255)                                                                   aacaacTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCaa  <  2:818657/81‑1 (MQ=255)                                                                   aacaacTTG‑AAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCaa  <  1:818656/81‑1 (MQ=255)                                                                            |                                                                         TGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAA  >  NC_000913/4060847‑4060994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑