| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 442,582 | Δ1 bp | coding (1034/1449 nt) | thiI → | tRNA uridine 4‑sulfurtransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 442,581 | 0 | G | . | 97.1% | 139.1 / ‑1.5 | 35 | coding (1033/1449 nt) | thiI | tRNA uridine 4‑sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (17/17); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
ACGACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTC > NC_000913/442504‑442655 | aCGACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATtgtg < 2:99316/78‑1 (MQ=255) gagCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCa < 1:99317/81‑1 (MQ=255) gCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCaa > 2:99366/1‑80 (MQ=255) catcatCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGc > 1:99375/1‑81 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGcc > 2:99360/1‑81 (MQ=255) catcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGccc > 2:99373/1‑80 (MQ=255) tcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGAc < 1:99318/81‑1 (MQ=255) aCCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGt < 2:99319/81‑1 (MQ=255) gCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGaaa < 1:99320/80‑1 (MQ=255) gCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAg > 1:99359/1‑81 (MQ=255) cccGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGc < 1:99321/81‑1 (MQ=255) cAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTa < 1:99322/81‑1 (MQ=255) aGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTa > 2:99374/1‑80 (MQ=255) gATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaaa < 2:99323/81‑1 (MQ=255) ttGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATc < 2:99324/81‑1 (MQ=255) tGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATc > 2:99377/1‑80 (MQ=255) gCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGa < 2:99325/80‑1 (MQ=255) cACCGAAGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCg > 2:99381/1‑78 (MQ=255) cACCGAAGACTTTGCTCGCACGAGGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAg < 1:99326/81‑1 (MQ=255) aaGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGa < 1:99327/81‑1 (MQ=255) aaGACTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGa < 1:99328/81‑1 (MQ=255) cTTTGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCg > 1:99379/1‑81 (MQ=255) tttGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCg > 2:99378/1‑80 (MQ=255) tttGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCg > 2:99371/1‑80 (MQ=255) ttGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCgg > 1:99368/1‑80 (MQ=255) tGCTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCgg > 1:865820/1‑79 (MQ=255) cTCGCACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:99329/80‑1 (MQ=255) cACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaagaa > 2:99383/1‑78 (MQ=255) cACGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaagaa > 2:99367/1‑78 (MQ=255) aCGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga < 1:99330/81‑1 (MQ=255) cGATGCCGGAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa < 1:99331/81‑1 (MQ=255) ggAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGAc > 2:99364/1‑81 (MQ=255) ggAATATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGAc > 1:99388/1‑81 (MQ=255) aaTATTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACtt < 2:99332/81‑1 (MQ=255) atatTGT‑GTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTc < 2:99333/81‑1 (MQ=255) tatTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTc < 2:99334/81‑1 (MQ=255) | ACGACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTC > NC_000913/442504‑442655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |