Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,504,957	(T)8→7	coding (900/1218 nt)	yjhB →	putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,504,950	0	T	.	100.0%	36.8 / NA	10	coding (893/1218 nt)	yjhB	putative sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (2/8);  total (2/8)

GTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAA  >  NC_000913/4504872‑4505006                                                                               |                                                         gTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                                                        <  2:932999/81‑1 (MQ=255)    ttgttgGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttttt                                                      <  2:933000/80‑1 (MQ=255)                 gATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGaaa                                       <  1:933001/81‑1 (MQ=255)                           aaaaaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAActcttct                              <  2:933002/80‑1 (MQ=255)                            aaaaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAActcttctct                            <  2:933003/81‑1 (MQ=255)                                  ccTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                      <  2:933004/81‑1 (MQ=255)                                   cTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGa                     <  2:933005/81‑1 (MQ=255)                                      tGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGAtt                   <  1:933006/80‑1 (MQ=255)                                          gTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc              >  1:933017/1‑81 (MQ=255)                                          gTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc              >  1:933022/1‑81 (MQ=255)                                               tCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt         <  2:933007/81‑1 (MQ=255)                                                      aCTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTaa  <  1:933008/81‑1 (MQ=255)                                                                               |                                                         GTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAA  >  NC_000913/4504872‑4505006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑