BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,520,574	(T)_5→4	intergenic (‑250/+97)	yjhU ← / ← yjhF	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU/KpLE2 phage‑like element; putative transporter YjhF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,520,570	0	T	.	100.0%	106.5 / NA	26	intergenic (‑246/+101)	yjhU/yjhF	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU/KpLE2 phage‑like element; putative transporter YjhF
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (16/10); total (16/10)

GGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAAC  >  NC_000913/4520496‑4520644

ggAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttgat                                                                      >  1:936876/1‑78 (MQ=255)

     cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCa                                                                <  2:936846/81‑1 (MQ=255)

     cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCa                                                                <  2:936845/81‑1 (MQ=255)

                 ttACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCAc                                                    >  2:936878/1‑81 (MQ=255)

                          tACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGAc                                           >  1:936883/1‑81 (MQ=255)

                            cTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACgg                                         >  1:936880/1‑81 (MQ=255)

                            cTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACgg                                         >  1:936874/1‑81 (MQ=255)

                              gagaATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGtt                                       >  1:936872/1‑81 (MQ=255)

                               agaATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGtt                                       <  1:936847/80‑1 (MQ=255)

                               agaATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTg                                      <  1:936848/81‑1 (MQ=255)

                                gaATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGc                                     >  2:936879/1‑81 (MQ=255)

                                     cacCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtca                                >  1:936875/1‑81 (MQ=255)

                                     cacCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtca                                >  2:936882/1‑81 (MQ=255)

                                     cacCCATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtc                                 <  1:936849/80‑1 (MQ=255)

                                       cccATCAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcact                              >  2:936881/1‑81 (MQ=255)

                                            cAAGCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTcactcact                          >  2:936885/1‑80 (MQ=255)

                                               gCATAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAAt                      <  2:936850/81‑1 (MQ=255)

                                                 aTAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTa                    >  2:936892/1‑81 (MQ=255)

                                                 aTAATCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTa                    >  2:936928/1‑81 (MQ=255)

                                                   aaTCGAAGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                  >  2:936888/1‑81 (MQ=255)

                                                        aaGGTTCAATATCGCCCC‑TTTTGATCGCCGGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGa             <  2:936851/81‑1 (MQ=255)

                                                         aGGTTCAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGa             >  1:936889/1‑80 (MQ=255)

                                                              cAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTg        <  2:936852/80‑1 (MQ=255)

                                                              cAATATCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTg        >  2:936884/1‑80 (MQ=255)

                                                              cAATATCGCCCC‑TTTTGATCGCCAGAGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTg        <  2:936853/80‑1 (MQ=255)

                                                                   tCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaacaac  <  1:936854/81‑1 (MQ=255)

                                                                   tCGCCCC‑TTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTAACTCACTAAATTATGGGGGACAGTGaacaa   >  1:936908/1‑80 (MQ=255)

                                                                     cgccccTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaacaa   >  2:936887/3‑79 (MQ=255)

GGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAAC  >  NC_000913/4520496‑4520644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`