Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,580,100	(C)5→4	coding (1363/1395 nt)	hsdS ←	type I restriction enzyme EcoKI specificity protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,580,096	0	C	.	100.0%	71.8 / NA	18	coding (1367/1395 nt)	hsdS	type I restriction enzyme EcoKI specificity protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/6);  total (12/6)

TTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGAT  >  NC_000913/4580016‑4580163                                                                                 |                                                                    ttGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTAc                                                                     >  1:950327/1‑81 (MQ=255)   ggCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTAccc                                                                   >  2:950335/1‑81 (MQ=255)    gCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTAcccc                                                                  <  2:950300/81‑1 (MQ=255)         aaaTAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAg                                                            <  1:950301/81‑1 (MQ=255)               taaAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTgcgc                                                      >  1:950321/1‑81 (MQ=255)                 aaaaGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGt                                                    >  2:950329/1‑81 (MQ=255)                       gcACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCt                                              <  1:950302/81‑1 (MQ=255)                          ccGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCttt                                            >  1:950377/1‑80 (MQ=255)                            ggAAAGGTTCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGAt                                         >  2:950324/1‑81 (MQ=255)                            ggAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGAt                                         <  1:950303/81‑1 (MQ=255)                             gAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGAtt                                        >  1:950341/1‑81 (MQ=255)                              aaaGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGAttt                                       >  2:950328/1‑81 (MQ=255)                                   tGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTc                                   >  1:950338/1‑80 (MQ=255)                                      gcCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGc                               <  1:950304/81‑1 (MQ=255)                                                         gATTTTTTACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGtttt            >  1:950340/1‑81 (MQ=255)                                                                tACGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGc      <  2:950305/80‑1 (MQ=255)                                                                 aCGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCGGCt     >  2:950349/1‑80 (MQ=255)                                                                 aCGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCt     <  2:950306/80‑1 (MQ=255)                                                                 aCGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCt     >  2:950339/1‑80 (MQ=255)                                                                 aCGTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCt     >  1:950330/1‑80 (MQ=255)                                                                   gTGAGGCTTTTTTA‑CCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGAt  >  1:950344/1‑81 (MQ=255)                                                                                 |                                                                    TTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGAT  >  NC_000913/4580016‑4580163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑