Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	451,589	Δ1 bp	coding (22/948 nt)	cyoA ←	cytochrome bo3 ubiquinol oxidase subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	451,586	0	T	.	100.0%	63.1 / NA	16	coding (25/948 nt)	cyoA	cytochrome bo3 ubiquinol oxidase subunit 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (5/11);  total (5/11)

CTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGT  >  NC_000913/451511‑451655                                                                            |                                                                      cTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACtttatt                                                                  <  1:101304/81‑4 (MQ=255) cTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACtttatt                                                                  >  1:101338/1‑78 (MQ=255)             aCAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCt                                                        <  2:101306/78‑1 (MQ=255)             aCAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGa                                                      <  2:101305/80‑1 (MQ=255)                  gcAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCa                                                <  2:101307/81‑1 (MQ=255)                               cACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCa                                   <  1:101308/81‑1 (MQ=255)                                aCTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCaa                                  >  1:101363/1‑81 (MQ=255)                                 cTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAAt                                 <  1:101309/81‑1 (MQ=255)                                      cAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCAc                            >  1:101339/1‑81 (MQ=255)                                           cAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGaa                       <  1:101311/81‑2 (MQ=255)                                           cAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGa                        <  2:101310/80‑1 (MQ=255)                                            aGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATc                      >  1:101343/1‑81 (MQ=255)                                             gTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCt                     <  1:101312/81‑1 (MQ=255)                                                 cTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtggtg                 <  2:101313/81‑1 (MQ=255)                                                        taatGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGttt           >  2:101345/1‑80 (MQ=255)                                                          atGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTaaa        <  1:101314/81‑1 (MQ=255)                                                             aCAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAgt      <  2:101315/80‑1 (MQ=255)                                                                aCCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAgtgtgt  >  1:101348/1‑81 (MQ=255)                                                                            |                                                                      CTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGT  >  NC_000913/451511‑451655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑