Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	515,325	(C)5→4	coding (449/918 nt)	qmcA ←	PHB domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	515,321	0	C	.	100.0%	93.6 / NA	23	coding (453/918 nt)	qmcA	PHB domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/14);  total (9/14)

TTTCATCTGCGCGTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAATCCCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCTGAGAGAGCATTTC  >  NC_000913/515243‑515395                                                                               |                                                                           tttCATCTGCGCGTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAATccc                                                                          >  2:114757/1‑81 (MQ=255)     aTCTGCGCGTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAATccccacg                                                                      >  2:114782/1‑78 (MQ=255)     aTCTGCGCGTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAATccccac                                                                       <  2:114729/80‑3 (MQ=255)         gcgcGTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGtt                                                                 >  1:114779/1‑81 (MQ=255)          cgcgTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGtt                                                                 <  2:114730/80‑1 (MQ=255)            cgTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTtggt                                                              <  2:114731/81‑1 (MQ=255)             gTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTtggtg                                                             >  2:114775/1‑81 (MQ=255)                     aaGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCAt                                                      <  2:114732/80‑1 (MQ=255)                      agagATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCg                                                    <  2:114733/81‑1 (MQ=255)                           tAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAAt                                               <  2:114734/81‑1 (MQ=255)                            aaGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATa                                              >  2:114783/1‑81 (MQ=255)                                    ccGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACgcagc                                        <  1:114735/79‑1 (MQ=255)                                    ccGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGcagcag                                      <  2:114736/81‑1 (MQ=255)                                      ggTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGc                                    <  1:114737/81‑1 (MQ=255)                                                 gTCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATg                         <  1:114738/81‑1 (MQ=255)                                                  tCGCGAATTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGc                        <  2:114739/81‑1 (MQ=255)                                                       aaTTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTcg                   <  2:114740/81‑1 (MQ=255)                                                       aaTTTCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTcg                   >  1:114778/1‑81 (MQ=255)                                                         tttCAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTcgcg                 >  2:114781/1‑81 (MQ=255)                                                            cAATACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCt               >  1:114785/1‑80 (MQ=255)                                                             aaTACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCTg              <  1:114741/80‑1 (MQ=255)                                                               tACGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCTga             >  1:114780/1‑79 (MQ=255)                                                                 cGGGTGACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCTgaga           >  1:114784/1‑79 (MQ=255)                                                                      gACTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCTGAGAGAGCAtt    >  2:114788/1‑81 (MQ=255)                                                                        cTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCTGAGAGAGCATTTc  <  2:114742/81‑1 (MQ=255)                                                                        cTTTAAT‑CCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCTGAGAGAGCATTTc  <  1:114743/81‑1 (MQ=255)                                                                               |                                                                           TTTCATCTGCGCGTTCATTGAAGAGATAAGCTCTGCCGGTGGGCGCACGTCGCGAATTTCAATACGGGTGACTTTAATCCCCCACGGGTTGGTGGCTTCATCGACAATACGCAGCAGGCGTGAGTTGATGCTGTCGCGCTGAGAGAGCATTTC  >  NC_000913/515243‑515395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑