Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	525,638	Δ1 bp	coding (2378/4281 nt)	rhsD →	protein RhsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	525,638	0	T	.	100.0%	135.7 / NA	33	coding (2378/4281 nt)	rhsD	protein RhsD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/15);  total (18/15)

CTGTGGCAGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTCGA  >  NC_000913/525568‑525713                                                                       |                                                                            ctgTGGCAGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTg                                                                   >  2:117084/1‑80 (MQ=255)   gtgGCAGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAg                                                                >  2:117078/1‑81 (MQ=255)     ggCAGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTg                                                              >  1:117096/1‑81 (MQ=255)     ggCAGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTg                                                              >  1:117080/1‑81 (MQ=255)          caTGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGa                                                         <  1:117025/81‑1 (MQ=255)             gagaCGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACg                                                       >  1:117079/1‑80 (MQ=255)              agaCGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGt                                                      <  1:117026/80‑1 (MQ=255)               gaCGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTAt                                                    >  2:117076/1‑81 (MQ=255)                aCGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATg                                                   <  1:117027/81‑1 (MQ=255)                  gAAACACGCATACAACGAGCAGGGGCTGGCAAGCCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATgg                                                  >  1:117073/1‑80 (MQ=18)                  gAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATgg                                                  >  2:117093/1‑80 (MQ=255)                  gAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATgg                                                  >  2:117083/1‑80 (MQ=255)                  gAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGc                                                 <  1:117029/81‑1 (MQ=255)                  gAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGc                                                 <  2:117028/81‑1 (MQ=255)                     acacGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGc                                              <  1:117030/81‑1 (MQ=255)                      cacGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCg                                             <  1:117031/81‑1 (MQ=255)                         gCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGtt                                          <  1:117032/81‑1 (MQ=255)                          cATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTa                                         >  2:117081/1‑81 (MQ=255)                           aTACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTAc                                        >  1:117105/1‑81 (MQ=255)                              cAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTg                                     >  1:117082/1‑81 (MQ=255)                               aaCGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTgg                                    >  2:117098/1‑81 (MQ=255)                                 cGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCg                                  <  1:117033/81‑1 (MQ=255)                                  gAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCgg                                 >  1:117164/1‑81 (MQ=255)                                  gAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCgg                                 >  2:117058/1‑81 (MQ=255)                                      aGGGGCTGGCAAACCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAAt                             <  2:117034/81‑1 (MQ=255)                                                aaaCCGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGc                   >  1:117090/1‑81 (MQ=255)                                                   ccGCGTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCggg                <  2:117035/81‑1 (MQ=255)                                                     gcgTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCGGGAc              <  1:117036/81‑1 (MQ=255)                                                       gTCACGCCGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCGGGAcgc            >  2:117101/1‑81 (MQ=255)                                                             ccGGACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTgg      <  1:117037/81‑1 (MQ=255)                                                               ggACAGCC‑GCCGCCGGTGGAGTGGCTGACGTGTGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTc    <  2:117038/81‑1 (MQ=255)                                                                gACAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTc    >  1:117102/1‑80 (MQ=255)                                                                 aCAGCC‑GCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTCGa  <  1:117039/81‑1 (MQ=255)                                                                       |                                                                            CTGTGGCAGCATGAGACGAAACACGCATACAACGAGCAGGGGCTGGCAAACCGCGTCACGCCGGACAGCCTGCCGCCGGTGGAGTGGCTGACGTATGGCAGCGGTTACCTGGCGGGAATGAAGCTGGGCGGGACGCCGCTGGTCGA  >  NC_000913/525568‑525713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑