| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 77,593 | (T)8→9 | noncoding (227/227 nt) | sgrS → | small regulatory RNA SgrS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 77,585 | 1 | . | T | 96.2% | 88.6 / ‑3.2 | 26 | noncoding (219/227 nt) | sgrS | small regulatory RNA SgrS |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (12/13); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTAC > NC_000913/77506‑77665 | aTTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑t < 2:18989/81‑1 (MQ=255) ttCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑tt < 2:18990/81‑1 (MQ=255) tCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑ttt > 2:19040/1‑81 (MQ=255) aaaGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑ttttttt < 1:18992/81‑1 (MQ=255) aaaGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑tttttt < 2:18991/80‑1 (MQ=255) ttGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑tttttttttat > 1:19036/1‑78 (MQ=255) gACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑tttttttttatt < 1:18993/80‑5 (MQ=255) cTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATtctc < 2:18994/81‑1 (MQ=255) ccTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTcgcc < 2:18995/80‑1 (MQ=255) cTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTcgccgc < 2:18996/81‑1 (MQ=255) catcatGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCcgcg < 2:18998/79‑1 (MQ=255) catcatGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCt < 2:18997/81‑1 (MQ=255) atcatGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCt > 1:19030/1‑80 (MQ=255) atcatGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTa < 2:18999/81‑1 (MQ=255) atGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTaaa > 1:19034/1‑80 (MQ=255) atGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTaaa < 2:19000/80‑1 (MQ=255) tgtgACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAgg > 1:19033/1‑81 (MQ=255) tGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACg > 1:19028/1‑80 (MQ=255) gAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTa > 1:19038/1‑81 (MQ=255) aGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTAt < 1:19001/81‑1 (MQ=255) aGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTAt > 1:19037/1‑81 (MQ=255) aaaaTCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATaa > 2:19041/1‑81 (MQ=255) aaaTCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAAt < 1:19002/80‑1 (MQ=255) aTCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAAGGa > 2:19042/1‑81 (MQ=255) tCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGAc < 2:19003/81‑1 (MQ=255) aCCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGa > 2:19029/1‑81 (MQ=255) ccAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCt < 2:19004/81‑1 (MQ=255) cagcagATTATACCTGCTGGTTTTTTTTTATTCTCGCCTCGCTAAAATTGGAACGTTTTTTCTTGATATTTACGTTGCCTc > 1:1013016/1‑81 (MQ=255) cagcagATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTc < 1:19005/81‑1 (MQ=255) ttATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTAt < 2:19006/81‑1 (MQ=255) ttATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTAt > 1:19032/1‑81 (MQ=255) ttATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTAt < 1:19007/81‑1 (MQ=255) ccTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACg > 2:19027/1‑81 (MQ=255) g‑TTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTAc < 2:19011/81‑1 (MQ=255) | ATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTAC > NC_000913/77506‑77665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |