| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 819,067 | (T)7→8 | coding (20/246 nt) | moaD → | molybdopterin synthase sulfur carrier subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 819,060 | 1 | . | T | 94.7% | 61.6 / ‑3.1 | 19 | coding (13/246 nt) | moaD | molybdopterin synthase sulfur carrier subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (9/9); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
ATTGGTCCGGTACGTTTGCTGGCGAAGAGCGGCGGCAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTC‑TTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGATTTCCCAACTGTTGAA > NC_000913/818984‑819134 | aTTGGTCCGGTACGTTTGCTGGCGAAGAGCGGCGGCAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTC‑tttt < 2:172780/81‑1 (MQ=255) ttGGTCCGGTACGTTTGCTGGCGAAGAGCGGCGGCAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTC‑ttttt < 2:172781/81‑1 (MQ=255) cGGTACGTTTGCTGGCGAAGAGCGGCGGCAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTC‑ttttttttcgc > 1:172831/1‑77 (MQ=255) gTACGTTTGCTGGCGAAGAGCGGCGGCAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGccc < 2:172782/81‑1 (MQ=255) tttGCTGGCGAAGAGCGGCGGCAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTg < 2:172783/81‑1 (MQ=255) gAAGAGCGGCGGCAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTtg > 2:172820/1‑81 (MQ=255) ggcggcAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGaa > 1:172832/1‑81 (MQ=255) aaGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCCAGTTGGTGGGAACAGATg > 1:172834/1‑81 (MQ=255) cGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAAc > 1:172839/1‑81 (MQ=255) gggTGACTTTAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAAcc < 1:172785/81‑1 (MQ=255) gggTGACTTTAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAAcc < 1:172784/81‑1 (MQ=255) gTGACTTTAAGGTGGAAGCGGATGATTAAAGTTC‑TTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGaa < 2:172786/81‑1 (MQ=255) ttAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGc > 2:172822/1‑81 (MQ=255) tAAGGTGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTgg < 1:172787/79‑1 (MQ=255) tGGAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGa < 1:172788/81‑1 (MQ=255) gAAGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGAtt > 2:172833/1‑81 (MQ=255) aaGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGAttt < 2:172789/81‑1 (MQ=255) aGCGGATGATTAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGATTTc > 2:172826/1‑81 (MQ=255) ttAAAGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGATTTCCCAACTGtt > 1:172823/1‑81 (MQ=255) aaGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGATTTCCCAACTGTTg > 1:172829/1‑79 (MQ=255) aaGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGATTTCCCAACTGTTGaa < 1:172790/81‑1 (MQ=255) aaGTTCTTTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGATTTCCCAACTGTTGa < 1:172791/80‑1 (MQ=255) | ATTGGTCCGGTACGTTTGCTGGCGAAGAGCGGCGGCAAGTCGGGTGACTTTAAGGTGGAAGCGGATGATTAAAGTTC‑TTTTTTTCGCCCAGGTGCGCGAGTTGGTGGGAACAGATGCAACCGAAGTGGCTGCGGATTTCCCAACTGTTGAA > NC_000913/818984‑819134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |