Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	926,165	(A)5→6	intergenic (‑194/+60)	serW ← / ← infA	tRNA‑Ser/translation initiation factor IF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	926,160	1	.	A	100.0%	69.5 / NA	20	intergenic (‑189/+65)	serW/infA	tRNA‑Ser/translation initiation factor IF‑1
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (11/9);  total (11/9)

TTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAAT‑AAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTA  >  NC_000913/926089‑926234                                                                         |                                                                           ttGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAAT‑aaaaaaggc                                                                   <  2:192220/81‑5 (MQ=255)    tgCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCg                                                                  <  2:192221/80‑1 (MQ=255)          atatCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAAc                                                           >  1:192257/1‑81 (MQ=255)           tatCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAAcc                                                          <  2:192222/81‑1 (MQ=255)           tatCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAAcc                                                          >  1:192252/1‑81 (MQ=255)            atCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAAcc                                                          >  1:192223/1‑80 (MQ=255)            atCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAAcc                                                          >  2:192248/1‑80 (MQ=255)                 cATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGa                                                        <  2:192224/77‑1 (MQ=255)                 cATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGAc                                                       <  1:192225/78‑1 (MQ=255)                  aTCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGACCttt                                                   <  1:192226/81‑1 (MQ=255)                  aTCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGACCttt                                                   <  1:192227/81‑1 (MQ=255)                     gagaCAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTAc                                                >  2:192275/1‑81 (MQ=255)                         cAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGt                                            >  1:192255/1‑81 (MQ=255)                            gTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGttct                                         <  1:192228/81‑1 (MQ=255)                                    cTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTc                                 <  2:192229/81‑1 (MQ=255)                                       ataCTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGcc                              >  1:192251/1‑81 (MQ=255)                                        taCTTCCAAAGTACTTCATACATATCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGcc                              <  1:192230/80‑1 (MQ=255)                                                           acataTCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAAt           >  2:192245/1‑80 (MQ=255)                                                            cataTCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAAt           >  1:192253/1‑79 (MQ=255)                                                             atatCACAAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAg        >  1:192247/1‑81 (MQ=255)                                                                   caAAATAAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTa  >  2:192260/1‑81 (MQ=255)                                                                         |                                                                           TTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAAT‑AAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTA  >  NC_000913/926089‑926234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑