| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,133,116 | (A)6→7 | coding (543/1209 nt) | flgE → | flagellar hook protein FlgE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,133,110 | 1 | . | A | 96.0% | 84.7 / ‑3.2 | 25 | coding (537/1209 nt) | flgE | flagellar hook protein FlgE |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (11/13); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CGATGCAGATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAAC‑AAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCG > NC_000913/1133031‑1133183 | cGATGCAGATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAAc > 1:227134/1‑80 (MQ=255) aaCCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTc > 1:227132/1‑81 (MQ=255) cTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGt > 1:227135/1‑81 (MQ=255) gAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGa > 2:227137/1‑81 (MQ=255) gAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGa > 1:227141/1‑81 (MQ=255) aaTTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTg < 2:227108/79‑1 (MQ=255) aaTTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGa < 1:227109/80‑1 (MQ=255) ccAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGt < 1:227110/80‑1 (MQ=255) cAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGtt < 2:227111/80‑1 (MQ=255) tGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCg < 1:227112/81‑1 (MQ=255) aTCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGAc < 2:227113/81‑1 (MQ=255) aTCCGCTTCCTACTGTTACACCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGa < 2:227114/80‑1 (MQ=255) cTTCCTACTGTTACGCCATTCAGCGCCAGCAACGCGGATGGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGt > 1:227143/1‑79 (MQ=255) ccTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAggg > 1:227140/1‑81 (MQ=255) tACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGt > 2:227142/1‑80 (MQ=255) cTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTa < 1:227115/79‑1 (MQ=255) cTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAAt > 1:227210/1‑81 (MQ=255) aCGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTc < 2:227116/80‑1 (MQ=255) aTTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTcatgaca < 2:227117/81‑1 (MQ=255) cAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACt < 2:227118/81‑1 (MQ=255) aTGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACttt < 2:227119/81‑1 (MQ=255) gCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTtgt > 2:227145/1‑81 (MQ=255) gCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTtgt > 2:227149/1‑81 (MQ=255) gATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTtgtg > 2:227144/1‑79 (MQ=255) aGCTATAAC‑AAAAACAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGAc > 2:227147/1‑81 (MQ=255) cTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCg < 1:227120/81‑1 (MQ=255) | CGATGCAGATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAAC‑AAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCG > NC_000913/1133031‑1133183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |