Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,762	(A)5→6	coding (651/1023 nt)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,757	1	.	A	100.0%	71.9 / NA	21	E216K (GAA→AAA)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (10/11);  total (10/11)

GATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCAT  >  NC_000913/1154679‑1154824                                                                                |                                                                    gATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCG‑a                                                                    >  2:231092/1‑80 (MQ=255)      cgcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCG‑aaaaaag                                                              <  1:231058/81‑3 (MQ=255)      cgcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCG‑aaaaaag                                                              <  2:231057/81‑3 (MQ=255)       gcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCG‑aaaaaag                                                              >  1:231102/1‑78 (MQ=255)           gggAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAAcc                                                          >  1:231096/1‑81 (MQ=255)              aaGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAAccgcc                                                       <  1:231061/81‑1 (MQ=255)                 ggCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGtt                                                    <  2:231064/81‑1 (MQ=255)                 ggCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGtt                                                    <  2:231062/81‑1 (MQ=255)                 ggCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGtt                                                    <  1:231063/81‑1 (MQ=255)                       cGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGt                                              >  2:231095/1‑81 (MQ=255)                           cGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAAc                                          >  2:231099/1‑81 (MQ=255)                                           gATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTc                          <  1:231065/81‑1 (MQ=255)                                             tAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAg                        <  2:231066/81‑1 (MQ=255)                                              aaaaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAg                        >  2:231103/1‑80 (MQ=255)                                                aaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTAt                     >  2:231100/1‑81 (MQ=255)                                                  aCCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTAttt                   <  1:231068/81‑1 (MQ=255)                                                  aCCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTAttt                   <  1:231067/81‑1 (MQ=255)                                                    cAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTAt                 <  1:231069/81‑1 (MQ=255)                                                      gTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCa               <  2:231070/81‑1 (MQ=255)                                                       ttGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCaa              <  2:231071/81‑1 (MQ=255)                                                        tGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAAc             >  1:231106/1‑81 (MQ=255)                                                            gACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGtt         >  2:231115/1‑81 (MQ=255)                                                            gACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGtt         >  2:231140/1‑81 (MQ=255)                                                                   gCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCAt  >  2:231098/1‑81 (MQ=255)                                                                   gCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCAt  >  2:231111/1‑81 (MQ=255)                                                                                |                                                                    GATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCAT  >  NC_000913/1154679‑1154824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑