Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,157,840	(A)7→8	intergenic (+266/‑29)	ycfH → / → ptsG	putative metal‑dependent hydrolase YcfH/glucose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,157,833	1	.	A	100.0%	50.0 / NA	15	intergenic (+259/‑36)	ycfH/ptsG	putative metal‑dependent hydrolase YcfH/glucose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (6/9);  total (6/9)

AAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGT‑AAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGT  >  NC_000913/1157773‑1157907                                                              |                                                                           aaGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTc                                                         <  2:231560/81‑1 (MQ=255)  aGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACt                                                          >  1:231586/1‑79 (MQ=255)  aGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCa                                                        <  1:231561/81‑1 (MQ=255)        cTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCa                                                  >  1:231600/1‑81 (MQ=255)        cTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCa                                                  >  2:231584/1‑81 (MQ=255)            gATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCActct                                              <  1:231563/81‑1 (MQ=255)            gATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCActct                                              <  2:231562/81‑1 (MQ=255)                 ccTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAAt                                          >  1:231587/1‑80 (MQ=255)                     tACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATg                                      <  2:231564/80‑1 (MQ=255)                       cacGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGtt                                    <  1:231565/80‑1 (MQ=255)                         cGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGttt                                   >  1:231589/1‑79 (MQ=255)                         cGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTaa                                 <  1:231566/81‑1 (MQ=255)                             gAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAAt                             <  2:231567/81‑1 (MQ=255)                                                   cgTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAgg       <  2:231568/81‑1 (MQ=255)                                                         aaCGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGt  >  1:231594/1‑80 (MQ=255)                                                              |                                                                           AAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGT‑AAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGT  >  NC_000913/1157773‑1157907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑