Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,229,560	(T)7→8	coding (835/912 nt)	hlyE ←	hemolysin E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,229,553	1	.	T	100.0%	42.6 / NA	13	M281K (ATG→AAG)	hlyE	hemolysin E
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (5/8);  total (5/8)

AATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCA  >  NC_000913/1229474‑1229624                                                                                 |                                                                        aatCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑t                                                                        >  1:242196/1‑81 (MQ=255)                   aGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGcttctt                                                       >  2:242193/1‑81 (MQ=255)                        ccTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGc                                                  <  1:242177/81‑1 (MQ=255)                        ccTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGc                                                  <  2:242176/81‑1 (MQ=255)                             aaGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCaaaga                                             <  2:242178/81‑1 (MQ=255)                              agagTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCaaaga                                             >  2:242198/1‑80 (MQ=255)                                   gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAt                                       >  2:242208/1‑81 (MQ=255)                                                          aTACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAg                 >  2:242195/1‑80 (MQ=255)                                                             cTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAAt              <  1:242179/80‑1 (MQ=255)                                                                aTTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCt            <  1:242180/79‑1 (MQ=255)                                                                aTTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTgg          <  1:242181/81‑1 (MQ=255)                                                                aTTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTgg          >  2:242199/1‑81 (MQ=255)                                                                   aCAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgtt        <  2:242182/80‑1 (MQ=255)                                                                        tgtTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCa  <  2:242183/81‑1 (MQ=255)                                                                                 |                                                                        AATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCA  >  NC_000913/1229474‑1229624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑