Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,418,441	(A)7→8	intergenic (‑212/‑230)	kilR ← / → sieB	Rac prophage; inhibitor of FtsZ, killing protein/Rac prophage; phage superinfection exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,418,434	1	.	A	100.0%	72.3 / NA	21	intergenic (‑205/‑237)	kilR/sieB	Rac prophage; inhibitor of FtsZ, killing protein/Rac prophage; phage superinfection exclusion protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (6/15);  total (6/15)

ACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAG‑AAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTT  >  NC_000913/1418355‑1418507                                                                                 |                                                                          aCTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTTAAAAGGGCGGACATCAGCCGAACTTCAAg                                                                            >  2:270899/1‑80 (MQ=255) aCTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAg                                                                            >  2:270908/1‑80 (MQ=255)      gCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAG‑aaaaa                                                                      >  1:270906/1‑80 (MQ=255)           gTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAG‑aaaaaaaact                                                                 >  2:270903/1‑77 (MQ=255)              cgcTTAGCCGCATTCACCGAATCACAAAATTCACTTTAAAAAGGGCGGACAGCAGCCGAACTTCAAGAAAAAAAACTGAt                                                               <  1:270877/80‑1 (MQ=25)               gcTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATgc                                                             <  2:270878/81‑1 (MQ=255)                cTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATgcc                                                            <  1:270879/81‑1 (MQ=255)                    gCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATgccgcc                                                         <  2:270880/80‑1 (MQ=255)                    gCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCa                                                        <  2:270881/81‑1 (MQ=255)                          ttCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTa                                                  >  2:270898/1‑81 (MQ=255)                               cGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTacaca                                              >  2:270904/1‑80 (MQ=255)                                gCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTAcacaca                                            <  1:270882/81‑1 (MQ=255)                                 cATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAg                                           <  1:270883/81‑1 (MQ=255)                                     acaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAAt                                       <  2:270884/81‑1 (MQ=255)                                           ttCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGtt                                 <  2:270885/81‑1 (MQ=255)                                                    aaaaGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAAc                        >  1:270902/1‑81 (MQ=255)                                                     aaaGGGCGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAAcc                       >  2:378692/1‑81 (MQ=255)                                                           cGGACATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGgc                 <  1:270886/81‑1 (MQ=255)                                                               cATCAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCAc             <  2:270887/81‑1 (MQ=255)                                                                  cAGCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTcc          >  1:270912/1‑81 (MQ=255)                                                                    gCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCcca        >  1:270910/1‑81 (MQ=255)                                                                    gCCGAACTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCcca        <  2:270888/81‑1 (MQ=255)                                                                         aCTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCatt   <  2:270889/81‑1 (MQ=255)                                                                          cTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCattt  <  1:270890/81‑1 (MQ=255)                                                                          cTTCAAGAAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCattt  <  2:270891/81‑1 (MQ=255)                                                                                 |                                                                          ACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAG‑AAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTT  >  NC_000913/1418355‑1418507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑