Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,844,593	(T)7→8	coding (279/1041 nt)	ynjI ←	DUF1266 domain‑containing protein YnjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,844,586	1	.	T	100.0%	41.0 / NA	13	E96K (GAA→AAA)	ynjI	DUF1266 domain‑containing protein YnjI
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (6/7);  total (6/7)

GGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTC‑TTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATAAAGAA  >  NC_000913/1844507‑1844645                                                                                 |                                                            ggTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTC‑t                                                            <  2:331415/81‑1 (MQ=255)     cGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTC‑ttttt                                                        <  1:331416/81‑1 (MQ=255)         cAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTC‑ttttttttg                                                    >  1:331437/1‑79 (MQ=255)              gTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCttt                                                 <  1:331417/80‑1 (MQ=255)               tAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTaa                                               <  2:331418/81‑1 (MQ=255)               tAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTa                                                <  2:331419/80‑1 (MQ=255)                        tAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGcc                                       <  1:331420/80‑1 (MQ=255)                              ctcGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGCCCGAAc                                  <  2:331421/79‑1 (MQ=255)                              ctcGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGCCCGAACaa                                >  1:331436/1‑81 (MQ=255)                                 gATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGCCCGAACAAt                               <  2:331422/79‑1 (MQ=255)                                   tACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGc                           >  2:331438/1‑81 (MQ=255)                                   tACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGACCGAACAATAAGc                           <  2:331423/81‑1 (MQ=255)                                           ggCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCcatca                    >  1:331444/1‑80 (MQ=255)                                                  tACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaattaa             >  1:331449/1‑80 (MQ=255)                                                         aGCCTGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATa       >  1:331445/1‑79 (MQ=255)                                                             tGATAATAATGCAGTTGTTCTTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATaaagaa  >  2:331443/1‑80 (MQ=255)                                                                                 |                                                            GGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTC‑TTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATAAAGAA  >  NC_000913/1844507‑1844645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑